Literature summary extracted from
Hirasawa, M.; Tripathy, J.; Sommer, F.; Somasundaram, R.; Chung, J.; Nestander, M.; Kruthiventi, M.; Zabet-Moghaddam, M.; Johnson, M.; Merchant, S.; Allen, J.; Knaff, D.
Enzymatic properties of the ferredoxin-dependent nitrite reductase from Chlamydomonas reinhardtii. Evidence for hydroxylamine as a late intermediate in ammonia production (2010), Photosynth. Res., 103, 67-77.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.7.7.1 |
expression in Escherichia coli |
Chlamydomonas reinhardtii |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.7.7.1 |
modeling of structure based on spinach nitrite reductase. Arginine and lysine residues are involved in electrostatically-stabilized binding to ferredoxin |
Chlamydomonas reinhardtii |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.7.7.1 |
63000 |
- |
x * 63000, SDS-PAGE |
Chlamydomonas reinhardtii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.7.7.1 |
Chlamydomonas reinhardtii |
A8J4P1 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.7.7.1 |
hydroxylamine + reduced ferredoxin |
hydroxylamine can serve as an electron-accepting substrate for the enzyme and the product of hydroxylamine reduction is ammonia. Hydroxylamine, bound to the enzyme, can serve as a late intermediate during the reduction of nitrite to ammonia catalyzed by the enzyme |
Chlamydomonas reinhardtii |
ammonia + H2O + oxidized ferredoxin |
- |
? |
|
1.7.7.1 |
additional information |
presence of two separate one-electron redox couples with Em values of -255 mV and -390 mV, respcetively |
Chlamydomonas reinhardtii |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.7.7.1 |
? |
x * 63000, SDS-PAGE |
Chlamydomonas reinhardtii |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.7.7.1 |
Nii1 |
- |
Chlamydomonas reinhardtii |