EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | jasmonic acid | - |
Brassica napus | |
1.4.1.2 | salicylic acid | - |
Brassica napus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.4.1.2 | mitochondrion | - |
Brassica napus | 5739 | - |
1.4.1.2 | protoplast | isozyme GDH7 predominates | Brassica napus | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | L-glutamate + H2O + NAD+ | Brassica napus | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.2 | Brassica napus | - |
GDH isoenzymes 1 and 7, genes GDH1 and GDH2 encoding subunits alpha and beta | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.1.2 | leaf | the isoenzyme profile in leaves changes on wounding | Brassica napus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.2 | L-glutamate + H2O + NAD+ | - |
Brassica napus | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.4.1.2 | hexamer | (alphabeta)3 | Brassica napus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.2 | NAD+-dependent GDH | - |
Brassica napus |
1.4.1.2 | NAD+-dependent glutamate dehydrogenase | - |
Brassica napus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.1.2 | NAD+ | - |
Brassica napus | |
1.4.1.2 | NADH | - |
Brassica napus |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.4.1.2 | Brassica napus | transcription of GDH2, which encodes the GDH alpha subunit, is activated and translation of the GDH2 mRNA is also activated to synthesize a subunit polypeptides, when protoplasts are isolated. When detached leaves absorb either acidic 5 mM jasmonic acid or salicylic acid solutions via petioles, GDH7 isoenzyme is activated and the GDH isoenzyme expression pattern is similar to that of protoplasts | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.1.2 | additional information | the isoenzyme profile in leaves changes on wounding, the change in GDH isoenzyme profile has no effect on ammonium assimilation. Protoplast isolation changes the redox state with NAD(P)H and oxidized glutathione levels increasing, and ascorbate, dehydroascorbate, NAD(P)+ and glutathione decreasing. ATP content in protoplasts declines to 2.6% of that in leaves, while that in wounded leaves increases by twofold | Brassica napus |
1.4.1.2 | physiological function | isozyme GDH7 does not support net amination in vivo and the increase in GDH7 activity might be a response to oxidative stress during protoplast isolation | Brassica napus |