Literature summary extracted from
Sun, L.; Sun, Y.; Zhang, M.; Wang, L.; Ren, J.; Cui, M.; Wang, Y.; Ji, K.; Li, P.; Li, Q.; Chen, P.; Dai, S.; Duan, C.; Wu, Y.; Leng, P.
Suppression of 9-cis-epoxycarotenoid dioxygenase, which encodes a key enzyme in abscisic acid biosynthesis, alters fruit texture in transgenic tomato (2012), Plant Physiol., 158, 283-298.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.13.11.51 |
gene SlNCED1, real-time quantitative reverse transcription, phylogenetic tree |
Solanum lycopersicum |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.51 |
additional information |
fruit-specific suppression of gene SlNCED1, avoid the pleiotropic phenotypes associated with abscisic acid deficiency, using fruit-specific E8 promoter in RNAi constructs, abscisic acid accumulation and SlNCED1 transcript levels in the transgenic fruit are downregulated to between 20% and 50% of the levels measured in the control fruit. Significant reduction in 9-cis-epoxycarotenoid dioxygenase activity leads to a downregulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase, pectin methyl esterase, beta-galactosidase precursor mRNA, xyloglucan endotransglycosylase, endo-1,4-beta cellulose, and expansin resulting in an increased accumulation of pectin during ripening and thus to significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45% |
Solanum lycopersicum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
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1.13.11.51 |
Solanum lycopersicum |
- |
gene SlNCED1 |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
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1.13.11.51 |
fruit |
- |
Solanum lycopersicum |
- |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
1.13.11.51 |
malfunction |
suppression of 9-cis-epoxycarotenoid dioxygenase alters fruit texture in transgenic tomato. Significant reduction in 9-cis-epoxycarotenoid dioxygenase activity leads to a downregulation in the transcription of genes encoding major cell wall catabolic enzymes, specifically polygalacturonase, pectin methyl esterase, beta-galactosidase precursor mRNA, xyloglucan endotransglycosylase, endo-1,4-beta cellulose, and expansin resulting in an increased accumulation of pectin during ripening and thus to significant extension of the shelf life to 15 to 29 d compared with a shelf life of only 7 d for the control fruit and an enhancement of fruit firmness at the mature stage by 30% to 45%, phenotypes, overview |
Solanum lycopersicum |
1.13.11.51 |
metabolism |
9-cis-epoxycarotenoid dioxygenase is a key enzyme in abscisic acid biosynthesis |
Solanum lycopersicum |