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Literature summary extracted from

  • Brodhun, F.; Cristobal-Sarramian, A.; Zabel, S.; Newie, J.; Hamberg, M.; Feussner, I.
    An iron 13S-lipoxygenase with an alpha-linolenic acid specific hydroperoxidase activity from Fusarium oxysporum (2013), PLoS ONE, 8, e64919.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.12 gene FoxLOX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparison, recombinnat expression of His-tagged enzyme Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.12 additional information
-
 additional information Michaelis-Menten kinetics Fusarium oxysporum
1.13.11.12 0.00337
-
 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 0.00689
-
 (9Z,12Z)-octadeca-9,12-dienoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 0.0316
-
 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 0.0348
-
 (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid pH 8.0, 22°C Fusarium oxysporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.12 Fe2+ weakly active site-bound, required for catalysis, non-heme iron lipoxygenase Fusarium oxysporum
1.13.11.12 additional information specifically binds iron but not manganese Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.12 8500
-
 2 * 8500, recombinant His-tagged enzyme, SDS-PAGE Fusarium oxysporum
1.13.11.12 160000
-
 about, recombinant His-tagged enzyme, gel filtration Fusarium oxysporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.12 linoleate + O2 Fusarium oxysporum
-
 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
1.13.11.12 linoleate + O2 Fusarium oxysporum 4287
-
 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
1.13.11.12 additional information Fusarium oxysporum product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview ?
-
 ?
1.13.11.12 additional information Fusarium oxysporum 4287 product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.12 Fusarium oxysporum
-
 f. sp. lycopersici
-
1.13.11.12 Fusarium oxysporum 4287
-
 f. sp. lycopersici
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.12 mycelium
-
 Fusarium oxysporum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.12 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
1.13.11.12 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid + O2
-
 Fusarium oxysporum 4287 ?
-
 ?
1.13.11.12 (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
1.13.11.12 (9Z,12Z)-octadeca-9,12-dienoic acid + O2
-
 Fusarium oxysporum ?
-
 ?
1.13.11.12 (9Z,12Z)-octadeca-9,12-dienoic acid + O2
-
 Fusarium oxysporum 4287 ?
-
 ?
1.13.11.12 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid + O2
-
 Fusarium oxysporum (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
 ?
1.13.11.12 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid + O2
-
 Fusarium oxysporum 4287 (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
 ?
1.13.11.12 linoleate + O2
-
 Fusarium oxysporum (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
1.13.11.12 linoleate + O2
-
 Fusarium oxysporum 4287 (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
 ?
1.13.11.12 additional information product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview Fusarium oxysporum ?
-
 ?
1.13.11.12 additional information product specificity of FoxLOX, by SP-HPLC/DAD analysis, overview Fusarium oxysporum 4287 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.12 homodimer 2 * 8500, recombinant His-tagged enzyme, SDS-PAGE Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
1.13.11.12 13-lipoxygenase
-
 Fusarium oxysporum
1.13.11.12 iron 13S-lipoxygenase
-
 Fusarium oxysporum
1.13.11.12 LOX
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.12 22
-
 assay at room temperaature Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.12 1.566
-
 (9Z,12Z,15Z)-9,12,15-octadecatrienoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 1.71
-
 (5Z,8Z,11Z,14Z)-eicosatetra-5,8,11,14-enoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 2.611
-
 (9Z,12Z)-octadeca-9,12-dienoic acid pH 8.0, 22°C Fusarium oxysporum
1.13.11.12 18.58
-
 (6Z,9Z,12Z)-octadeca-6,9,12-trienoic acid pH 8.0, 22°C Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.12 8
-
-
 Fusarium oxysporum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.12 7 10.5 activity range, overview. Low activity below pH 7.0, maximum activity at pH 8.0, 75% of maximal activity at up to pH 10.5 Fusarium oxysporum

General Information

EC Number General Information Comment Organism
1.13.11.12 additional information non-heme iron lipoxygenase oxidizes C18-polyunsaturated fatty acids to 13S-hydroperoxy derivatives by an antarafacial reaction mechanism where the bis-allylic hydrogen abstraction is the rate-limiting step Fusarium oxysporum