Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bari, M.R.; Hassan, M.; Akai, N.; Arima, J.; Mori, N.
    Gene cloning and biochemical characterization of 4-N-trimethylaminobutyraldehyde dehydrogenase II from Pseudomonas sp. 13CM (2013), World J. Microbiol. Biotechnol., 29, 683-692.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.47 expressed in Escherichia coli DH5alpha and BL21 (DE3) cells Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.47 4-chloromercuribenzoate
-
 Pseudomonas sp.
1.2.1.47 5,5'-dithiobis-(2-nitrobenzoic acid)
-
 Pseudomonas sp.
1.2.1.47 Ba2+
-
 Pseudomonas sp.
1.2.1.47 Co2+
-
 Pseudomonas sp.
1.2.1.47 Cu2+
-
 Pseudomonas sp.
1.2.1.47 Hg2+
-
 Pseudomonas sp.
1.2.1.47 iodoacetoamide
-
 Pseudomonas sp.
1.2.1.47 K+
-
 Pseudomonas sp.
1.2.1.47 Li+
-
 Pseudomonas sp.
1.2.1.47 additional information potassium fluoride, sodium azide, 2,2'-dipyridyl, and EDTA have no effect on the enzyme activity Pseudomonas sp.
1.2.1.47 Semicarbazide
-
 Pseudomonas sp.
1.2.1.47 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.47 0.31
-
 4-N-trimethylaminobutyraldehyde at pH 9.5 and 30°C Pseudomonas sp.
1.2.1.47 0.62
-
 4-dimethylaminobutyraldehyde at pH 9.5 and 30°C Pseudomonas sp.
1.2.1.47 1.16
-
 NAD+ at pH 9.5 and 30°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.47 additional information Rb+, Na+, Mg2+, and Ca2+ have no effect on the enzyme activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.47 52000
-
 3 * 52000, SDS-PAGE Pseudomonas sp.
1.2.1.47 150000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.47 4-N-trimethylaminobutyraldehyde + NAD+ + H2O Pseudomonas sp.
-
 gamma-butyrobetaine + NADH + H+
-
 ?
1.2.1.47 4-N-trimethylaminobutyraldehyde + NAD+ + H2O Pseudomonas sp. 13CM
-
 gamma-butyrobetaine + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.47 Pseudomonas sp. N0DT23
-
-
1.2.1.47 Pseudomonas sp. 13CM N0DT23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.47 ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and 5'-AMP Sepharose column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.47 0.8
-
 cell free extract, at pH 9.5 and 30°C Pseudomonas sp.
1.2.1.47 12.4
-
 native enzyme, after 19.5fold purification, at pH 9.5 and 30°C Pseudomonas sp.
1.2.1.47 15.4
-
 recombinant enzyme, after 19.5fold purification, at pH 9.5 and 30°C Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.47 4-dimethylaminobutyraldehyde + NAD+ + H2O
-
 Pseudomonas sp. ? + NADH + H+
-
 ?
1.2.1.47 4-dimethylaminobutyraldehyde + NAD+ + H2O
-
 Pseudomonas sp. 13CM ? + NADH + H+
-
 ?
1.2.1.47 4-N-trimethylaminobutyraldehyde + NAD+ + H2O
-
 Pseudomonas sp. gamma-butyrobetaine + NADH + H+
-
 ?
1.2.1.47 4-N-trimethylaminobutyraldehyde + NAD+ + H2O
-
 Pseudomonas sp. 13CM gamma-butyrobetaine + NADH + H+
-
 ?
1.2.1.47 additional information no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. ?
-
 ?
1.2.1.47 additional information no activity with NADP+, trimethylaminobutanol, 3-N-trimethylaminopropionaldehyde, betaine aldehyde, 4-aminobutyraldehyde, and other aliphatic aldehydes such as acetaldehyde, propionaldehyde, butyraldehyde, pivaleraldehyde, isovaleraldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. 13CM ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.47 homotrimer 3 * 52000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.1.47 4-N-trimethylaminobutyraldehyde dehydrogenase II
-
 Pseudomonas sp.
1.2.1.47 TMABaldehyde-DH II
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.47 40
-
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.47 25 30 the enzyme is stable up to 25°C while incubation for 30 min at 30°C and above leads to rapid inactivation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.47 9.5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.47 8 10 after pre-incubation for 15 min at 30°C, the enzyme is stable between pH 8.0 and 9.0 but is inactivated completely at pH 10.0 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.47 NAD+
-
 Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.47 Pseudomonas sp. isoelectric focusing
-
 4.5