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Literature summary extracted from

  • Rokov-Plavec, J.; Lesjak, S.; Gruic-Sovulj, I.; Mocibob, M.; Dulic, M.; Weygand-Durasevic, I.
    Substrate recognition and fidelity of maize seryl-tRNA synthetases (2013), Arch. Biochem. Biophys., 529, 122-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 expressed in Escherichia coli strain KL229 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 0.0008628
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 0.18
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 2 3.4 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 2.35
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 29
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.11 cytosol the enzyme is localized exclusively in the cytosol Zea mays 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASer Zea mays
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 additional information Zea mays the enzyme shows pre-transfer editing activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + DL-serine hydroxamate + tRNASer very weak activity Zea mays ?
-
 ?
6.1.1.11 ATP + L-cysteine + tRNASer very weak activity Zea mays AMP + diphosphate + L-cysteinyl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer
-
 Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-serine + tRNASer tRNASer from Escherichia coli Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.11 ATP + L-threonine + tRNASer very weak activity Zea mays AMP + diphosphate + L-threonyl-tRNASer
-
 ?
6.1.1.11 additional information the enzyme shows pre-transfer editing activity Zea mays ?
-
 ?
6.1.1.11 additional information Gly73 is a major identity element recognized by the enzyme Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 SerRS
-
 Zea mays
6.1.1.11 Seryl-tRNA synthetase
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.11 0.078
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 0.14
-
 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 1.1
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 2.433
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 2.5
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
 Zea mays

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.11 0.0027
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 0.006
-
 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 1.04
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 13.9
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
6.1.1.11 1275
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays