Any feedback?
Literature summary extracted from
- Characterization of a thermophilic L-arabinose isomerase from Thermoanaerobacterium saccharolyticum NTOU1 (2014), Biochem. Eng. J., 83, 121-128.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermoanaerobacterium saccharolyticum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | Dulcitol | 90% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum |  |
| 5.3.1.4 | erythritol | 42% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
| 5.3.1.4 | L-arabitol | 11% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
| 5.3.1.4 | ribitol | 22% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
| 5.3.1.4 | xylitol | 46% residual activity at 10 mM | Thermoanaerobacterium saccharolyticum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 122 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | Co2+ | 0.05 mM Co2+, can improve both catalytic activity and thermostability at higher temperatures | Thermoanaerobacterium saccharolyticum | |
| 5.3.1.4 | Mn2+ | 0.1 mM Mn2+,increases activity to 167.3% and can improve both catalytic activity and thermostability at higher temperatures | Thermoanaerobacterium saccharolyticum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 52863 | - |
4 * 52863, native enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | 55000 | - |
4 * 55000, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | 55343 | - |
4 * 55343, recombinant enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | 55397 | - |
4 * 55397, recombinant enzyme, MALDI-TOF mass spectrometry | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | 233000 | - |
gel filtration | Thermoanaerobacterium saccharolyticum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | D-fucose | Thermoanaerobacterium saccharolyticum | 221% activity compared to D-galactose | D-fuculose | - |
r | |
| 5.3.1.4 | D-galactose | Thermoanaerobacterium saccharolyticum | 100% activity | D-tagatose | - |
r | |
| 5.3.1.4 | L-arabinose | Thermoanaerobacterium saccharolyticum | 244% activity compared to D-galactose | L-ribulose | - |
r | |
| 5.3.1.4 | L-arabinose | Thermoanaerobacterium saccharolyticum NTOU1 | 244% activity compared to D-galactose | L-ribulose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.4 | Thermoanaerobacterium saccharolyticum | K7SW59 | - |
- |
| 5.3.1.4 | Thermoanaerobacterium saccharolyticum NTOU1 | K7SW59 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | Ni-NTA column chromatography | Thermoanaerobacterium saccharolyticum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | D-Allose | 9% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | D-Allose | 9% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
| 5.3.1.4 | D-fucose | 221% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | D-fuculose | - |
r | |
| 5.3.1.4 | D-galactose | 100% activity | Thermoanaerobacterium saccharolyticum | D-tagatose | - |
r | |
| 5.3.1.4 | D-rhamnose | 28% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | D-Ribose | 7% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | D-Ribose | 7% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
| 5.3.1.4 | D-sorbose | 31% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | D-Xylose | 14% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | D-Xylose | 14% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
| 5.3.1.4 | L-arabinose | 244% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum | L-ribulose | - |
r | |
| 5.3.1.4 | L-arabinose | 244% activity compared to D-galactose | Thermoanaerobacterium saccharolyticum NTOU1 | L-ribulose | - |
r | |
| 5.3.1.4 | additional information | no activity with D-glucose, D-mannose, and L-fucose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| 5.3.1.4 | additional information | no activity with D-glucose, D-mannose, and L-fucose | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | homotetramer | 4 * 55000, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | homotetramer | 4 * 52863, native enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | homotetramer | 4 * 55343, recombinant enzyme, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | homotetramer | 4 * 55397, recombinant enzyme, MALDI-TOF mass spectrometry | Thermoanaerobacterium saccharolyticum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | AraA | - |
Thermoanaerobacterium saccharolyticum |
| 5.3.1.4 | L-AI | - |
Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 70 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 40 | 80 | the activity increases with the increasing temperature (starting with 22% activity at 40°C, 50% activity at 50°C), reaches an optimal activity at 70°C, and then drops with a steep slope down (65% activity at 75°C) to inactivity at 80°C | Thermoanaerobacterium saccharolyticum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 55 | 75 | the activity is fully retained after 2 h incubation at 55-65°C. In the presence of 0.1 mM Mn2+ and 0.05 mM Co2+, the enzyme can remain stable for 2 h during the incubation at 55-65°C, and the half-life of the enzyme is about 150 and 15 min at 70 and 75°C, respectively | Thermoanaerobacterium saccharolyticum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 4.9 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 7 | 7.5 | - |
Thermoanaerobacterium saccharolyticum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 5 | 9.5 | very minimal activity when the pH either drops below 5.0 or increases to above 9.5 | Thermoanaerobacterium saccharolyticum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 5.5 | 10.5 | remains stable after 24 h. The enzyme activity is fully retained at pH 6.5-9.5 after 6 h with about 70% remaining at pH 5.5 and 10.5 | Thermoanaerobacterium saccharolyticum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 0.04 | - |
D-galactose | in 25 mM Tris-HCl buffer (pH 7.5), at 60°C | Thermoanaerobacterium saccharolyticum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
