EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.9 | expressed in Escherichia coli BL21(DE3) Rosetta cells | Helicobacter pylori |
3.2.2.16 | expressed in Escherichia coli BL21 (DE3) Rosetta cells | Helicobacter pylori |
3.2.2.30 | expressed in Escherichia coli BL21(DE3) Rosetta cells | Helicobacter pylori |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.9 | hanging drop vapor diffusion method. Inactive D198N mutant bound to S-adenosyl-L-homocysteine and active enzyme bound to S-(5-deoxy-D-ribos-5-yl)-L-homocysteine and adenine using 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES (pH 7.5), and 30% (v/v) PEG-MME 550 | Helicobacter pylori |
3.2.2.16 | hanging drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M HEPES pH 7.5 and 25% (w/v) PEG 3350 or 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5 and 30% (v/v) PEG-MME 550 | Helicobacter pylori |
3.2.2.30 | hanging drop vapor diffusion method. Inactive D198N mutant bound to 5'-methylthioadenosine using 0.2 M magnesium chloride, 0.1 M HEPES (pH 7.5), and 25% (w/v) PEG 3350 | Helicobacter pylori |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.2.16 | D198N | inactive | Helicobacter pylori |
3.2.2.16 | F107A | the mutant shows reduced activity compared to the wild type enzyme | Helicobacter pylori |
3.2.2.16 | H109A | the mutant shows reduced activity compared to the wild type enzyme | Helicobacter pylori |
3.2.2.30 | D198N | inactive | Helicobacter pylori |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.01 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 0.014 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 0.019 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.16 | 0.01 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.014 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.019 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.033 | - |
S-methyl-5'-thioadenosine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.037 | - |
S-methyl-5'-thioadenosine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.039 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | Helicobacter pylori | - |
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.16 | 5'-deoxyadenosine + H2O | Helicobacter pylori | - |
5-deoxy-D-ribose + adenine | - |
? | |
3.2.2.16 | 6-amino-6-deoxyfutalosine + H2O | Helicobacter pylori | - |
3-{3-[(2R,3S,4R,5R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl}benzoic acid + 9H-purine-6-amine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | Helicobacter pylori | - |
S-ribosyl-L-homocysteine + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | Helicobacter pylori | - |
S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | Helicobacter pylori | - |
dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.30 | additional information | Helicobacter pylori | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.9 | Helicobacter pylori | - |
- |
- |
3.2.2.16 | Helicobacter pylori | Q9ZMY2 | - |
- |
3.2.2.30 | Helicobacter pylori | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.9 | HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
3.2.2.16 | HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
3.2.2.30 | HisTrap column chromatography and Superdex 200 gel filtration | Helicobacter pylori |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.9 | S-adenosyl-L-homocysteine + H2O | - |
Helicobacter pylori | S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine | - |
? | |
3.2.2.16 | 5'-deoxyadenosine + H2O | - |
Helicobacter pylori | 5-deoxy-D-ribose + adenine | - |
? | |
3.2.2.16 | 6-amino-6-deoxyfutalosine + H2O | - |
Helicobacter pylori | 3-{3-[(2R,3S,4R,5R)-3,4,5-trihydroxytetrahydrofuran-2-yl]propanoyl}benzoic acid + 9H-purine-6-amine | - |
? | |
3.2.2.16 | S-adenosyl-L-homocysteine + H2O | - |
Helicobacter pylori | S-ribosyl-L-homocysteine + adenine | - |
? | |
3.2.2.16 | S-methyl-5'-thioadenosine + H2O | - |
Helicobacter pylori | S-methyl-5-thio-D-ribose + adenine | - |
? | |
3.2.2.30 | 6-amino-6-deoxyfutalosine + H2O | - |
Helicobacter pylori | dehypoxanthine futalosine + adenine | - |
? | |
3.2.2.30 | additional information | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase is a multifunctional enzyme that catalyzes the hydrolysis of the N-ribosidic bond of at least four different adenosine-based metabolites: S-adenosyl-L-homocysteine, 5'-methylthioadenosine, 5'-deoxyadenosine, and 6-amino-6-deoxyfutalosine | Helicobacter pylori | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.9 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
3.2.2.9 | MTAN | - |
Helicobacter pylori |
3.2.2.16 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
3.2.2.16 | MTAN | - |
Helicobacter pylori |
3.2.2.30 | 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase | - |
Helicobacter pylori |
3.2.2.30 | MTAN | - |
Helicobacter pylori |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 0.44 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 0.74 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 1.8 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.16 | 0.44 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 0.74 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 1 | - |
S-methyl-5'-thioadenosine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 1.2 | - |
S-methyl-5'-thioadenosine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 1.8 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 3.8 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.2.9 | 23 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 51 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.9 | 170 | - |
S-adenosyl-L-homocysteine | wild type enzyme, in 100 mM HEPES and 50 mM KCl buffer (pH 7.2), at 37°C | Helicobacter pylori | |
3.2.2.16 | 23 | - |
S-adenosyl-L-homocysteine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 27 | - |
S-methyl-5'-thioadenosine | mutant enzyme F107A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 37 | - |
S-methyl-5'-thioadenosine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 51 | - |
S-adenosyl-L-homocysteine | mutant enzyme H109A, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 97 | - |
S-methyl-5'-thioadenosine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori | |
3.2.2.16 | 170 | - |
S-adenosyl-L-homocysteine | wild type enzyme, at pH 7.2 and 25°C | Helicobacter pylori |