Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhai, X.; Amyes, T.L.; Wierenga, R.K.; Loria, J.P.; Richard, J.P.
    Structural mutations that probe the interactions between the catalytic and dianion activation sites of triosephosphate isomerase (2013), Biochemistry, 52, 5928-5940.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.1.1 phosphite
-
 Gallus gallus
5.3.1.1 phosphite
-
 Trypanosoma brucei brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expressed in Escherichia coli BL21 pLys S cells Trypanosoma brucei brucei
5.3.1.1 expressed in Escherichia coli FB215471(DE3) cells Gallus gallus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.1 L7RM the mutant exhibits a 200fold decrease in kcat/Km for isomerization of D-glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The mutant exhibits a 25fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme. The mutation has little effect on the observed and intrinsic phosphodianion binding energy and only a modest effect on phosphite dianion activation of the enzyme Gallus gallus
5.3.1.1 P168A the mutation results in 50fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme Trypanosoma brucei brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 2-Phosphoglycolate competitive inhibition Gallus gallus
5.3.1.1 2-Phosphoglycolate competitive inhibition Trypanosoma brucei brucei
5.3.1.1 arsenate competitive inhibition Gallus gallus
5.3.1.1 arsenate competitive inhibition Trypanosoma brucei brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.091
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 0.25
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 0.27
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 0.29
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 0.49
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 0.59
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 0.7
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 4
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate Gallus gallus
-
 dihydroxyacetone phosphate
-
 r
5.3.1.1 D-glyceraldehyde 3-phosphate Trypanosoma brucei brucei
-
 dihydroxyacetone phosphate
-
 r
5.3.1.1 dihydroxyacetone phosphate Gallus gallus
-
 D-glyceraldehyde 3-phosphate
-
 r
5.3.1.1 dihydroxyacetone phosphate Trypanosoma brucei brucei
-
 D-glyceraldehyde 3-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Gallus gallus
-
-
-
5.3.1.1 Trypanosoma brucei brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 CM Sepharose column chromatography Trypanosoma brucei brucei
5.3.1.1 DEAE-Sepharose column chromatography Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.1 muscle
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Gallus gallus dihydroxyacetone phosphate
-
 r
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Trypanosoma brucei brucei dihydroxyacetone phosphate
-
 r
5.3.1.1 dihydroxyacetone phosphate
-
 Gallus gallus D-glyceraldehyde 3-phosphate
-
 r
5.3.1.1 dihydroxyacetone phosphate
-
 Trypanosoma brucei brucei D-glyceraldehyde 3-phosphate
-
 r
5.3.1.1 glycolaldehyde
-
 Gallus gallus ?
-
 ?
5.3.1.1 glycolaldehyde
-
 Trypanosoma brucei brucei ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TIM
-
 Gallus gallus
5.3.1.1 TIM
-
 Trypanosoma brucei brucei
5.3.1.1 Triosephosphate isomerase
-
 Gallus gallus
5.3.1.1 Triosephosphate isomerase
-
 Trypanosoma brucei brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 6.5
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 8
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 16
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 24
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 300
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 340
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 2100
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 3200
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.1 0.019
-
 2-Phosphoglycolate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 0.055
-
 2-Phosphoglycolate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 0.14
-
 2-Phosphoglycolate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 2.3
-
 2-Phosphoglycolate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 3.8
-
 arsenate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 4.6
-
 arsenate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 9.6
-
 arsenate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 10
-
 arsenate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.1 2
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 13
-
 dihydroxyacetone phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 59
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 260
-
 D-glyceraldehyde 3-phosphate mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 430
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 580
-
 dihydroxyacetone phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus
5.3.1.1 8400
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Trypanosoma brucei brucei
5.3.1.1 11000
-
 D-glyceraldehyde 3-phosphate wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C Gallus gallus