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Literature summary extracted from

  • Quartararo, C.E.; Hadi, T.; Cahill, S.M.; Blanchard, J.S.
    Solvent isotope-induced equilibrium perturbation for isocitrate lyase (2013), Biochemistry, 52, 9286-9293.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 recombinant expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.045
-
 isocitrate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
4.1.3.1 0.24
-
 succinate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
4.1.3.1 0.4
-
 glyoxylate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Mycobacterium tuberculosis
-
 succinate + glyoxylate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Mycobacterium tuberculosis succinate + glyoxylate
-
 r
4.1.3.1 isocitrate isotopic analysis, solvent kinetic isotope effects in the direction of isocitrate cleavage arise from the initial deprotonation of the C2 hydroxyl group of isocitrate or the protonation of the aci-acid of the succinate product of the isocitrate aldolcleavage by a solvent-derived proton, equilibrium perturbation in D2O, NMR spectroscopy and mass spectrometry, modeling, overview. The final equilibrium isotopic composition of reactants in D2O reveals dideuterated succinate, protiated glyoxylate, and monodeuterated isocitrate, with the transient appearance and disappearance of monodeuterated succinate Mycobacterium tuberculosis succinate + glyoxylate
-
 r
4.1.3.1 succinate + glyoxylate
-
 Mycobacterium tuberculosis isocitrate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 ICL
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.1 25
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.1 3.6
-
 succinate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
4.1.3.1 3.6
-
 glyoxylate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis
4.1.3.1 5.2
-
 isocitrate pH 7.5, 25°C, recombinant enzyme Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7.5
-
 assay at Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
4.1.3.1 metabolism first enzyme of the glyoxylate shunt, which allows for the anaplerosis of citric acid cycle intermediates under nutrient limiting conditions Mycobacterium tuberculosis