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Literature summary extracted from

  • Ivanov, I.; Di Venere, A.; Horn, T.; Scheerer, P.; Nicolai, E.; Stehling, S.; Richter, C.; Skrzypczak-Jankun, E.; Mei, G.; Maccarrone, M.; Kuehn, H.
    Tight association of N-terminal and catalytic subunits of rabbit 12/15-lipoxygenase is important for protein stability and catalytic activity (2011), Biochim. Biophys. Acta, 1811, 1001-1010.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expressed in Escherichia coli Oryctolagus cuniculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 Y98A kcat and Km (linoleic acid) decreased compared to wild-type, mutant shows strongly reduced catalytic activity compared to wild-type Oryctolagus cuniculus
1.13.11.31 Y98F kcat and Km (linoleic acid) increased compared to wild-type Oryctolagus cuniculus
1.13.11.31 Y98R mutant shows strongly reduced catalytic activity compared to wild-type, mutant does not follow Michaelis-Menten-kinetics. Mutant is strongly inhibited by linoleic acid at concentrations above 0.01 mM Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.0125
-
 linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
1.13.11.31 0.0169
-
 linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
1.13.11.31 0.0213
-
 linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Oryctolagus cuniculus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 linoleic acid + O2
-
 Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12/15-lipoxygenases
-
 Oryctolagus cuniculus
1.13.11.31 12/15-LOX
-
 Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.31 14.7
-
 linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
1.13.11.31 44.2
-
 linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
1.13.11.31 65.2
-
 linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.31 1170
-
 linoleic acid pH 7.4, 20°, mutant Y98A Oryctolagus cuniculus
1.13.11.31 2600
-
 linoleic acid pH 7.4, 20°, wild-type Oryctolagus cuniculus
1.13.11.31 3060
-
 linoleic acid pH 7.4, 20°, mutant Y98F Oryctolagus cuniculus