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Literature summary extracted from
- Characterization of a recombinant thermostable D-lyxose isomerase from Dictyoglomus turgidum that produces D-lyxose from D-xylulose (2012), Biotechnol. Lett., 34, 1079-1085.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.15 | expressed in Escherichia coli ER2566 cells | Dictyoglomus turgidum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.15 | Ba2+ | - |
Dictyoglomus turgidum |  |
| 5.3.1.15 | EDTA | - |
Dictyoglomus turgidum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.15 | 1 | 3 | D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 5 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 39 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 65 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.15 | Co2+ | maximum activity (6.6fold) in the presence of 0.5 mM Co2+ | Dictyoglomus turgidum | |
| 5.3.1.15 | Mn2+ | stimulates activity but to a lesser extent than Co2+ | Dictyoglomus turgidum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.15 | 22000 | - |
2 * 22000, SDS-PAGE | Dictyoglomus turgidum |
| 5.3.1.15 | 44000 | - |
gel filtration | Dictyoglomus turgidum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.15 | D-Lyxose | Dictyoglomus turgidum | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | D-Xylulose | - |
? | |
| 5.3.1.15 | D-Lyxose | Dictyoglomus turgidum DSM 6724 | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | D-Xylulose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.15 | Dictyoglomus turgidum | B8DZQ9 | - |
- |
| 5.3.1.15 | Dictyoglomus turgidum DSM 6724 | B8DZQ9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.15 | metal ion affinity column chromatography | Dictyoglomus turgidum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.15 | 0.008 | - |
after 38fold purification, using L-allose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.022 | - |
after 38fold purification, using D-tagatose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.077 | - |
after 38fold purification, using D-ribulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.094 | - |
after 38fold purification, using D-fructose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.106 | - |
after 38fold purification, using D-talose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.119 | - |
after 38fold purification, using L-ribose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.292 | - |
after 38fold purification, using L-ribulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 0.5 | - |
crude extract, using D-lyxose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 1.668 | - |
after 38fold purification, using D-mannose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 18.8 | - |
after 38fold purification, using D-lyxose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
| 5.3.1.15 | 33.1 | - |
after 38fold purification, using D-xylulose as substrate, at pH 7.5 and 75°C | Dictyoglomus turgidum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.15 | D-fructose | - |
Dictyoglomus turgidum | D-mannose | - |
r | |
| 5.3.1.15 | D-Lyxose | - |
Dictyoglomus turgidum | D-Xylulose | - |
? | |
| 5.3.1.15 | D-Lyxose | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | Dictyoglomus turgidum | D-Xylulose | - |
? | |
| 5.3.1.15 | D-Lyxose | - |
Dictyoglomus turgidum DSM 6724 | D-Xylulose | - |
? | |
| 5.3.1.15 | D-Lyxose | highest specific activity, the enzyme converts 500 g D-xylulose/l to 380 g D-lyxose/l after 2 h | Dictyoglomus turgidum DSM 6724 | D-Xylulose | - |
? | |
| 5.3.1.15 | D-Mannose | - |
Dictyoglomus turgidum | D-Fructose | - |
r | |
| 5.3.1.15 | D-Mannose | - |
Dictyoglomus turgidum DSM 6724 | D-Fructose | - |
r | |
| 5.3.1.15 | D-ribulose | - |
Dictyoglomus turgidum | D-ribose | - |
r | |
| 5.3.1.15 | D-tagatose | - |
Dictyoglomus turgidum | D-talose | - |
r | |
| 5.3.1.15 | D-talose | - |
Dictyoglomus turgidum | D-tagatose | - |
r | |
| 5.3.1.15 | D-talose | - |
Dictyoglomus turgidum DSM 6724 | D-tagatose | - |
r | |
| 5.3.1.15 | D-xylulose | - |
Dictyoglomus turgidum | D-lyxose | - |
r | |
| 5.3.1.15 | L-allose | - |
Dictyoglomus turgidum | L-psicose | - |
r | |
| 5.3.1.15 | L-ribose | - |
Dictyoglomus turgidum | L-ribulose | - |
r | |
| 5.3.1.15 | L-ribose | - |
Dictyoglomus turgidum DSM 6724 | L-ribulose | - |
r | |
| 5.3.1.15 | L-ribulose | - |
Dictyoglomus turgidum | L-ribose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.15 | homodimer | 2 * 22000, SDS-PAGE | Dictyoglomus turgidum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.15 | 75 | - |
- |
Dictyoglomus turgidum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.15 | 60 | 85 | at pH 7.5 and 75°C and in the presence of 0.5 mM Co2+ the enzyme has a half-life of 108 min. After incubation at 75°C for 5 min, more than 97% of the enzyme activity remains. The half-lives of the enzyme at 60, 65, 70, 75, 80 and 85°C are 547, 247, 171, 108, 49, and 16 min, respectively | Dictyoglomus turgidum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.15 | 1.7 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 2.97 | - |
D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 59.5 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 69.3 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.15 | 7.5 | - |
- |
Dictyoglomus turgidum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.15 | 0.03 | - |
L-ribulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 0.23 | - |
D-mannose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 1.52 | - |
D-Lyxose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
| 5.3.1.15 | 15.3 | - |
D-xylulose | at pH 7.5 and 75°C | Dictyoglomus turgidum | |
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