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Literature summary extracted from

  • Zhang, S.; Li, W.; Zhang, D.; Huang, X.; Qin, W.; Gu, J.
    Purification and characterization of a low-temperature ammonia monooxygenase from heterotrophic nitrifier Acinetobacter sp. Y16 (2013), Desalin. Water Treatment, 53, 257-262.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.39 membrane bound Acinetobacter sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.39 CuCl2 stimulates enzyme activity in vitro Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.39 31000
-
 1 * 31000, SDS-PAGE Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.39 Acinetobacter sp.
-
-
-
1.14.99.39 Acinetobacter sp. Y16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.39 native enzyme by anion exchange chromatography and gel filtration Acinetobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.14.99.39 10°C, purified native enzyme, 2 days, stable Acinetobacter sp.
1.14.99.39 15°C, purified native enzyme, 1 day, stable Acinetobacter sp.
1.14.99.39 4°C, purified native enzyme, 5 days, stable Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.39 additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp. ?
-
 ?
1.14.99.39 additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp. Y16 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.39 monomer 1 * 31000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.99.39 AMO
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.99.39 15
-
 the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.99.39 4 20 activity range, inactive below and above Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.99.39 4 15 purified native enzyme, stable, over 91% remaining activity Acinetobacter sp.
1.14.99.39 20
-
 purified native enzyme, inactivation Acinetobacter sp.

General Information

EC Number General Information Comment Organism
1.14.99.39 additional information the enzyme can catalyze the oxidation of ammonium without stabilizing agents in vitro at low temperatures Acinetobacter sp.