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Literature summary extracted from

  • Kojima, H.; Moll, J.; Kahnt, J.; Fukui, M.; Shima, S.
    A reversed genetic approach reveals the coenzyme specificity and other catalytic properties of three enzymes putatively involved in anaerobic oxidation of methane with sulfate (2014), Environ. Microbiol., 16, 3431-3442.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.98.1 expression in Escherichia coli uncultured archaeon
2.3.1.101 expression in Escherichia coli uncultured archaeon
3.5.4.27 expression in Escherichia coli uncultured archaeon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.98.1 0.005
-
oxidized coenzyme F420 pH 6.0, 20°C uncultured archaeon
1.5.98.1 0.005
-
5,10-methylenetetrahydromethanopterin pH 6.0, 20°C uncultured archaeon
1.5.98.1 0.015
-
5,10-methylenetetrahydromethanopterin pH 6.0, 20°C uncultured archaeon
2.3.1.101 0.01
-
formylmethanofuran pH 6.0, 10°C uncultured archaeon
2.3.1.101 0.08
-
5,6,7,8-tetrahydromethanopterin pH 6.0, 10°C uncultured archaeon
3.5.4.27 0.06
-
5,10-methenyl-5,6,7,8-tetrahydromethanopterin pH 7.6, 10°C uncultured archaeon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.27 K+ activity in cell extract is dependent on the presence of high concentrations of potassium phosphate. The activity in 1.0 M potassium phosphate buffer pH 7.6 is eightfold higher than that in 0.12 M potassium phosphate buffer pH 7.6 uncultured archaeon

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.98.1 30283
-
x * 30283, calculated uncultured archaeon
1.5.98.1 30287
-
x * 30287, calculated from sequence uncultured archaeon
2.3.1.101 31864
-
x * 31864, calculated from sequence uncultured archaeon
3.5.4.27 34982
-
x * 34982, calculated from sequence uncultured archaeon

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin uncultured archaeon
-
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O uncultured archaeon
-
5-formyl-5,6,7,8-tetrahydromethanopterin
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.98.1 uncultured archaeon D1JH91
-
-
2.3.1.101 uncultured archaeon D1J8Z9
-
-
3.5.4.27 uncultured archaeon D1JGU3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.98.1
-
uncultured archaeon
2.3.1.101
-
uncultured archaeon
3.5.4.27
-
uncultured archaeon

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.98.1 1100
-
pH 6.0, 20°C uncultured archaeon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.98.1 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420
-
uncultured archaeon 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
-
uncultured archaeon methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
2.3.1.101 formylmethanofuran + 5,6,7,8-tetrahydromethanopterin the reverse reaction is difficult to measure because the free-energy change of the reverse direction is close to equilibrium but endergonic. Therefore, the reverse reaction stopps almost as soon as a small amount of substrate is converted to product uncultured archaeon methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
-
?
3.5.4.27 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O
-
uncultured archaeon 5-formyl-5,6,7,8-tetrahydromethanopterin
-
r

Subunits

EC Number Subunits Comment Organism
1.5.98.1 ? x * 30283, calculated uncultured archaeon
1.5.98.1 ? x * 30287, calculated from sequence uncultured archaeon
2.3.1.101 ? x * 31864, calculated from sequence uncultured archaeon
3.5.4.27 ? x * 34982, calculated from sequence uncultured archaeon

Synonyms

EC Number Synonyms Comment Organism
1.5.98.1 CBH37899
-
uncultured archaeon
1.5.98.1 F420-dependent methylenetetrahydromethanopterin dehydrogenase
-
uncultured archaeon
1.5.98.1 MTD
-
uncultured archaeon
2.3.1.101 CBH38579
-
uncultured archaeon
2.3.1.101 formylmethanofuran:tetrahydromethanopterin formyltransferase
-
uncultured archaeon
2.3.1.101 FTR
-
uncultured archaeon
3.5.4.27 CBH37751
-
uncultured archaeon
3.5.4.27 Mch
-
uncultured archaeon

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.98.1 70
-
-
uncultured archaeon
2.3.1.101 40
-
-
uncultured archaeon
3.5.4.27 50
-
-
uncultured archaeon

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.5.98.1 20 80 20°C: about 55% of maximal activity, 80°C: about 75% of maximal activity uncultured archaeon
2.3.1.101 20 50 20°C: about 60% of maximal activity, 50°C: about 60% of maximal activity uncultured archaeon
3.5.4.27 30 70 30°C: about 75% of maximal activity, 70°C: about 55% of maximal activity uncultured archaeon

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.98.1 10
-
enzyme diluted in 120 mM potassium phosphate buffer pH 6.0 at temperature below 10°C shows a half-life of less than 1 h. Higher concentrations of potassium phosphate stabilize, the optimum concentration being 0.5-0.8 M uncultured archaeon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.98.1 6
-
assay at uncultured archaeon
3.5.4.27 7.6
-
assay at uncultured archaeon

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.98.1 additional information when NAD+ or NADP+ is used instead of F420, the specific activity is less than 0.001 U/mg uncultured archaeon

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.98.1 uncultured archaeon calculated from sequence
-
6.1
2.3.1.101 uncultured archaeon calculated from sequence
-
4.7
3.5.4.27 uncultured archaeon calculated from sequence
-
4.3