EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.98.1 | expression in Escherichia coli | uncultured archaeon |
2.3.1.101 | expression in Escherichia coli | uncultured archaeon |
3.5.4.27 | expression in Escherichia coli | uncultured archaeon |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.98.1 | 0.005 | - |
oxidized coenzyme F420 | pH 6.0, 20°C | uncultured archaeon | |
1.5.98.1 | 0.005 | - |
5,10-methylenetetrahydromethanopterin | pH 6.0, 20°C | uncultured archaeon | |
1.5.98.1 | 0.015 | - |
5,10-methylenetetrahydromethanopterin | pH 6.0, 20°C | uncultured archaeon | |
2.3.1.101 | 0.01 | - |
formylmethanofuran | pH 6.0, 10°C | uncultured archaeon | |
2.3.1.101 | 0.08 | - |
5,6,7,8-tetrahydromethanopterin | pH 6.0, 10°C | uncultured archaeon | |
3.5.4.27 | 0.06 | - |
5,10-methenyl-5,6,7,8-tetrahydromethanopterin | pH 7.6, 10°C | uncultured archaeon |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.27 | K+ | activity in cell extract is dependent on the presence of high concentrations of potassium phosphate. The activity in 1.0 M potassium phosphate buffer pH 7.6 is eightfold higher than that in 0.12 M potassium phosphate buffer pH 7.6 | uncultured archaeon |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 30283 | - |
x * 30283, calculated | uncultured archaeon |
1.5.98.1 | 30287 | - |
x * 30287, calculated from sequence | uncultured archaeon |
2.3.1.101 | 31864 | - |
x * 31864, calculated from sequence | uncultured archaeon |
3.5.4.27 | 34982 | - |
x * 34982, calculated from sequence | uncultured archaeon |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | uncultured archaeon | - |
methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
3.5.4.27 | 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | uncultured archaeon | - |
5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.98.1 | uncultured archaeon | D1JH91 | - |
- |
2.3.1.101 | uncultured archaeon | D1J8Z9 | - |
- |
3.5.4.27 | uncultured archaeon | D1JGU3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.98.1 | - |
uncultured archaeon |
2.3.1.101 | - |
uncultured archaeon |
3.5.4.27 | - |
uncultured archaeon |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 1100 | - |
pH 6.0, 20°C | uncultured archaeon |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.98.1 | 5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | - |
uncultured archaeon | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | - |
uncultured archaeon | methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
2.3.1.101 | formylmethanofuran + 5,6,7,8-tetrahydromethanopterin | the reverse reaction is difficult to measure because the free-energy change of the reverse direction is close to equilibrium but endergonic. Therefore, the reverse reaction stopps almost as soon as a small amount of substrate is converted to product | uncultured archaeon | methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
? | |
3.5.4.27 | 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O | - |
uncultured archaeon | 5-formyl-5,6,7,8-tetrahydromethanopterin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.98.1 | ? | x * 30283, calculated | uncultured archaeon |
1.5.98.1 | ? | x * 30287, calculated from sequence | uncultured archaeon |
2.3.1.101 | ? | x * 31864, calculated from sequence | uncultured archaeon |
3.5.4.27 | ? | x * 34982, calculated from sequence | uncultured archaeon |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.98.1 | CBH37899 | - |
uncultured archaeon |
1.5.98.1 | F420-dependent methylenetetrahydromethanopterin dehydrogenase | - |
uncultured archaeon |
1.5.98.1 | MTD | - |
uncultured archaeon |
2.3.1.101 | CBH38579 | - |
uncultured archaeon |
2.3.1.101 | formylmethanofuran:tetrahydromethanopterin formyltransferase | - |
uncultured archaeon |
2.3.1.101 | FTR | - |
uncultured archaeon |
3.5.4.27 | CBH37751 | - |
uncultured archaeon |
3.5.4.27 | Mch | - |
uncultured archaeon |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 70 | - |
- |
uncultured archaeon |
2.3.1.101 | 40 | - |
- |
uncultured archaeon |
3.5.4.27 | 50 | - |
- |
uncultured archaeon |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 20 | 80 | 20°C: about 55% of maximal activity, 80°C: about 75% of maximal activity | uncultured archaeon |
2.3.1.101 | 20 | 50 | 20°C: about 60% of maximal activity, 50°C: about 60% of maximal activity | uncultured archaeon |
3.5.4.27 | 30 | 70 | 30°C: about 75% of maximal activity, 70°C: about 55% of maximal activity | uncultured archaeon |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 10 | - |
enzyme diluted in 120 mM potassium phosphate buffer pH 6.0 at temperature below 10°C shows a half-life of less than 1 h. Higher concentrations of potassium phosphate stabilize, the optimum concentration being 0.5-0.8 M | uncultured archaeon |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.98.1 | 6 | - |
assay at | uncultured archaeon |
3.5.4.27 | 7.6 | - |
assay at | uncultured archaeon |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.98.1 | additional information | when NAD+ or NADP+ is used instead of F420, the specific activity is less than 0.001 U/mg | uncultured archaeon |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.5.98.1 | uncultured archaeon | calculated from sequence | - |
6.1 |
2.3.1.101 | uncultured archaeon | calculated from sequence | - |
4.7 |
3.5.4.27 | uncultured archaeon | calculated from sequence | - |
4.3 |