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Literature summary extracted from

  • Zirngibl, C.; Van Dongen, W.; Schwörer, B.; Von Bünau, R.; Richter, M.; Klein, A.; Thauer, R.K.
    H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea (1992), Eur. J. Biochem., 208, 511-520.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.2
-
Methanopyrus kandleri

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.98.2 cyanide 1 mM, 50% inhibition Methanothermobacter marburgensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.2 additional information
-
H2 apparent Km for H2 is 60% Methanothermobacter wolfeii
1.12.98.2 additional information
-
H2 the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase Methanothermobacter marburgensis
1.12.98.2 0.02
-
5,10-methylenetetrahydromethanopterin pH and temperature not specified in the publication Methanothermobacter wolfeii
1.12.98.2 0.04
-
5,10-methylenetetrahydromethanopterin pH 6.5, 60°C Methanothermobacter marburgensis
1.12.98.2 0.05
-
5,10-methenyltetrahydromethanopterin pH 7.5, 60°C Methanothermobacter marburgensis
1.12.98.2 0.07
-
5,10-methenyltetrahydromethanopterin pH and temperature not specified in the publication Methanothermobacter wolfeii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.2 additional information the enzyme does not contain iron-sulfur clusters, nickel, or flavins Methanothermobacter marburgensis
1.12.98.2 Zn2+ the zinc content varies from preparation to preparation between 0.5-4 mol zinc/mol enzyme Methanothermobacter marburgensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.2 43000
-
-
Methanothermobacter marburgensis
1.12.98.2 43000
-
2 * 43000, SDS-PAGE Methanothermobacter wolfeii
1.12.98.2 92000
-
-
Methanothermobacter wolfeii

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.2 Methanopyrus kandleri Q02394
-
-
1.12.98.2 Methanopyrus kandleri DSM 6324 Q02394
-
-
1.12.98.2 Methanothermobacter marburgensis P32440
-
-
1.12.98.2 Methanothermobacter marburgensis DSM 2133 P32440
-
-
1.12.98.2 Methanothermobacter wolfeii P32441
-
-
1.12.98.2 Methanothermobacter wolfeii DSM 2970 P32441
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.2 rapidly inactivated in the presence of molecular oxygen. The rate of inactivation increases in the presence of dithiothreitol (2 mM) or of mercaptoethanol (5 mM) Methanothermobacter marburgensis
1.12.98.2 the enzyme is much more sensitive towards inactivation by O2 than the enzyme from Methanothermobacter marburgensis Methanothermobacter wolfeii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.2
-
Methanothermobacter marburgensis
1.12.98.2
-
Methanothermobacter wolfeii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.2 1250
-
pH 6, 60°C Methanothermobacter marburgensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.2 H+ + 5,10-methylenetetrahydromethanopterin
-
Methanothermobacter wolfeii H2 + 5,10-methenyltetrahydromethanopterin
-
r
1.12.98.2 H+ + 5,10-methylenetetrahydromethanopterin the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides Methanothermobacter marburgensis H2 + 5,10-methenyltetrahydromethanopterin
-
r
1.12.98.2 H+ + 5,10-methylenetetrahydromethanopterin
-
Methanothermobacter wolfeii DSM 2970 H2 + 5,10-methenyltetrahydromethanopterin
-
r
1.12.98.2 H+ + 5,10-methylenetetrahydromethanopterin the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides Methanothermobacter marburgensis DSM 2133 H2 + 5,10-methenyltetrahydromethanopterin
-
r

Subunits

EC Number Subunits Comment Organism
1.12.98.2 dimer 2 * 43000, SDS-PAGE Methanothermobacter wolfeii
1.12.98.2 homodimer
-
Methanothermobacter marburgensis

Synonyms

EC Number Synonyms Comment Organism
1.12.98.2 H2-forming methylenetetrahydromethanopterin dehydrogenase
-
Methanothermobacter marburgensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.2 60
-
in 120 mM potassium phosphate pH 6 Methanothermobacter marburgensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.98.2 60
-
30 min, 50% inactivation Methanothermobacter marburgensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.98.2 975
-
5,10-methenyltetrahydromethanopterin pH 7.5, 60°C Methanothermobacter marburgensis
1.12.98.2 1462
-
5,10-methylenetetrahydromethanopterin pH 6.5, 60°C Methanothermobacter marburgensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.2 6.5
-
-
Methanothermobacter marburgensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.12.98.2 Methanothermobacter marburgensis isoelectric focussing
-
4.4