EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.98.2 | - |
Methanopyrus kandleri |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.2 | cyanide | 1 mM, 50% inhibition | Methanothermobacter marburgensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.98.2 | additional information | - |
H2 | apparent Km for H2 is 60% | Methanothermobacter wolfeii | |
1.12.98.2 | additional information | - |
H2 | the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase | Methanothermobacter marburgensis | |
1.12.98.2 | 0.02 | - |
5,10-methylenetetrahydromethanopterin | pH and temperature not specified in the publication | Methanothermobacter wolfeii | |
1.12.98.2 | 0.04 | - |
5,10-methylenetetrahydromethanopterin | pH 6.5, 60°C | Methanothermobacter marburgensis | |
1.12.98.2 | 0.05 | - |
5,10-methenyltetrahydromethanopterin | pH 7.5, 60°C | Methanothermobacter marburgensis | |
1.12.98.2 | 0.07 | - |
5,10-methenyltetrahydromethanopterin | pH and temperature not specified in the publication | Methanothermobacter wolfeii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.2 | additional information | the enzyme does not contain iron-sulfur clusters, nickel, or flavins | Methanothermobacter marburgensis | |
1.12.98.2 | Zn2+ | the zinc content varies from preparation to preparation between 0.5-4 mol zinc/mol enzyme | Methanothermobacter marburgensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.98.2 | 43000 | - |
- |
Methanothermobacter marburgensis |
1.12.98.2 | 43000 | - |
2 * 43000, SDS-PAGE | Methanothermobacter wolfeii |
1.12.98.2 | 92000 | - |
- |
Methanothermobacter wolfeii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.98.2 | Methanopyrus kandleri | Q02394 | - |
- |
1.12.98.2 | Methanopyrus kandleri DSM 6324 | Q02394 | - |
- |
1.12.98.2 | Methanothermobacter marburgensis | P32440 | - |
- |
1.12.98.2 | Methanothermobacter marburgensis DSM 2133 | P32440 | - |
- |
1.12.98.2 | Methanothermobacter wolfeii | P32441 | - |
- |
1.12.98.2 | Methanothermobacter wolfeii DSM 2970 | P32441 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.98.2 | rapidly inactivated in the presence of molecular oxygen. The rate of inactivation increases in the presence of dithiothreitol (2 mM) or of mercaptoethanol (5 mM) | Methanothermobacter marburgensis |
1.12.98.2 | the enzyme is much more sensitive towards inactivation by O2 than the enzyme from Methanothermobacter marburgensis | Methanothermobacter wolfeii |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.98.2 | - |
Methanothermobacter marburgensis |
1.12.98.2 | - |
Methanothermobacter wolfeii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.98.2 | 1250 | - |
pH 6, 60°C | Methanothermobacter marburgensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.2 | H+ + 5,10-methylenetetrahydromethanopterin | - |
Methanothermobacter wolfeii | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
1.12.98.2 | H+ + 5,10-methylenetetrahydromethanopterin | the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides | Methanothermobacter marburgensis | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
1.12.98.2 | H+ + 5,10-methylenetetrahydromethanopterin | - |
Methanothermobacter wolfeii DSM 2970 | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
1.12.98.2 | H+ + 5,10-methylenetetrahydromethanopterin | the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides | Methanothermobacter marburgensis DSM 2133 | H2 + 5,10-methenyltetrahydromethanopterin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.98.2 | dimer | 2 * 43000, SDS-PAGE | Methanothermobacter wolfeii |
1.12.98.2 | homodimer | - |
Methanothermobacter marburgensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.12.98.2 | H2-forming methylenetetrahydromethanopterin dehydrogenase | - |
Methanothermobacter marburgensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.2 | 60 | - |
in 120 mM potassium phosphate pH 6 | Methanothermobacter marburgensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.2 | 60 | - |
30 min, 50% inactivation | Methanothermobacter marburgensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.98.2 | 975 | - |
5,10-methenyltetrahydromethanopterin | pH 7.5, 60°C | Methanothermobacter marburgensis | |
1.12.98.2 | 1462 | - |
5,10-methylenetetrahydromethanopterin | pH 6.5, 60°C | Methanothermobacter marburgensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.2 | 6.5 | - |
- |
Methanothermobacter marburgensis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.12.98.2 | Methanothermobacter marburgensis | isoelectric focussing | - |
4.4 |