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Literature summary extracted from

  • Ohshima, T.; Nunoura-Kominato, N.; Kudome, T.; Sakuraba, H.
    A novel hyperthermophilic archaeal glyoxylate reductase from Thermococcus litoralis. Characterization, gene cloning, nucleotide sequence and expression in Escherichia coli (2001), Eur. J. Biochem., 268, 4740-4747.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.26 cloned and expressed in Escherichia coli Thermococcus litoralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.26 4-chloromercuribenzoate 1 mM, 95% inhibition Thermococcus litoralis
1.1.1.26 CuCl2 1 mM, 66% inhibition Thermococcus litoralis
1.1.1.26 dithiothreitol 1 mM, 50% inhibition Thermococcus litoralis
1.1.1.26 HgCl2 1 mM, complete inhibition Thermococcus litoralis
1.1.1.26 additional information a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.26 0.067
-
 NADH pH 6.5, 50°C Thermococcus litoralis
1.1.1.26 0.73
-
 glyoxylate pH 6.5, 50°C Thermococcus litoralis
1.1.1.26 1.3
-
 Hydroxypyruvate pH 6.5, 50°C Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.26 36807
-
 2 * 36807, calculated from sequence Thermococcus litoralis
1.1.1.26 37000
-
 2 * 37000, SDS-PAGE Thermococcus litoralis
1.1.1.26 76000
-
 non-denaturimg gradient PAGE Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.26 glycolate + NAD+ Thermococcus litoralis
-
 glyoxylate + NADH + H+
-
 ?
1.1.1.26 glycolate + NAD+ Thermococcus litoralis DSM 5473
-
 glyoxylate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Thermococcus litoralis Q9C4M5
-
-
1.1.1.26 Thermococcus litoralis DSM 5473 Q9C4M5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.26
-
 Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.26 44.5
-
 pH 6.5, 50°C Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 glycolate + NAD+
-
 Thermococcus litoralis glyoxylate + NADH + H+
-
 ?
1.1.1.26 glycolate + NAD+
-
 Thermococcus litoralis DSM 5473 glyoxylate + NADH + H+
-
 ?
1.1.1.26 glyoxylate + NADH + H+ NADPH exhibits 7.5% of the activity compared to NADH Thermococcus litoralis glycolate + NAD+
-
 ?
1.1.1.26 glyoxylate + NADH + H+ NADPH exhibits 7.5% of the activity compared to NADH Thermococcus litoralis DSM 5473 glycolate + NAD+
-
 ?
1.1.1.26 hydroxypyruvate + NADH + H+ the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor Thermococcus litoralis D-glycerate + NAD+
-
 ?
1.1.1.26 hydroxypyruvate + NADH + H+ the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor Thermococcus litoralis DSM 5473 D-glycerate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.26 homodimer 2 * 37000, SDS-PAGE Thermococcus litoralis
1.1.1.26 homodimer 2 * 36807, calculated from sequence Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.26 NADH-dependent glyoxylate reductase
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.26 50
-
 assay at Thermococcus litoralis
1.1.1.26 90
-
-
 Thermococcus litoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.26 90
-
 10 min, stable up to Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.26 6.5
-
-
 Thermococcus litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NADH NADPH exhibits rather low activity as an electron donor compared with NADH Thermococcus litoralis