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Literature summary extracted from

  • Chen, Y.H.; Maity, A.N.; Pan, Y.C.; Frey, P.A.; Ke, S.C.
    Radical stabilization is crucial in the mechanism of action of lysine 5,6-aminomutase: role of tyrosine-263alpha as revealed by electron paramagnetic resonance spectroscopy (2011), J. Am. Chem. Soc., 133, 17152-17155.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.3 Y263F the mutation abolishes the enzymatic activity Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.3 (3S)-3,6-diaminohexanoate Acetoanaerobium sticklandii
-
 (3S,5S)-3,5-diaminohexanoate
-
 r
5.4.3.3 D-lysine Acetoanaerobium sticklandii
-
 2,5-diaminohexanoate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.3 Acetoanaerobium sticklandii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.3.3 (3S)-3,6-diaminohexanoate = (3S,5S)-3,5-diaminohexanoate radical stabilization is crucial in the mechanism of action of lysine 5,6-aminomutase, role of Tyr263alpha. The enzyme utilizes free radical intermediates to mediate 1,2-amino group rearrangement, during which an elusive high-energy aziridincarbinyl radical is proposed to be central in the mechanism of action. The aziridincarbinyl radical acts either as a spin-relay device or serves as an anchor for the pyridine ring of pyridoxal-5' -phosphate through aromatic PI-stacking interactions during spin transfer, detailed overview Acetoanaerobium sticklandii
a
5.4.3.3 D-lysine = (2R,5S)-2,5-diaminohexanoate radical stabilization is crucial in the mechanism of action of lysine 5,6-aminomutase, role of Tyr263alpha. The enzyme utilizes free radical intermediates to mediate 1,2-amino group rearrangement, during which an elusive high-energy aziridincarbinyl radical is proposed to be central in the mechanism of action. The aziridincarbinyl radical acts either as a spin-relay device or serves as an anchor for the pyridine ring of pyridoxal-5' -phosphate through aromatic PI-stacking interactions during spin transfer, detailed overview Acetoanaerobium sticklandii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.3 (3S)-3,6-diaminohexanoate
-
 Acetoanaerobium sticklandii (3S,5S)-3,5-diaminohexanoate
-
 r
5.4.3.3 D-lysine
-
 Acetoanaerobium sticklandii 2,5-diaminohexanoate
-
 r
5.4.3.3 additional information the enzyme can accept D-lysine and L-beta-lysine Acetoanaerobium sticklandii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.3.3 tetramer alpha2beta2 Acetoanaerobium sticklandii

Synonyms

EC Number Synonyms Comment Organism
5.4.3.3 5,6-LAM
-
 Acetoanaerobium sticklandii
5.4.3.3 lysine 5,6-aminomutase
-
 Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.3 5'-deoxyadenosylcobalamin dependent on Acetoanaerobium sticklandii
5.4.3.3 pyridoxal 5'-phosphate dependent on Acetoanaerobium sticklandii