EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.124 | the lovA gene in C-terminal FLAG-tagged form is cloned in the same vector coding cpr (cytochrome P450 oxidoreductase), and both lovA and cpr are co-expressed in Saccharomyces cerevisiae | Aspergillus terreus |
1.14.14.125 | the lovA gene in C-terminal FLAG-tagged form is cloned in the same vector coding cpr (cytochrome P450 oxidoreductase), and both lovA and cpr are co-expressed in Saccharomyces cerevisiae | Aspergillus terreus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.125 | 0.0062 | - |
monacolin L acid | pH and temperature not specified in the publication | Aspergillus terreus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.124 | dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus terreus | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.125 | monacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus terreus | monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid. The enzyme also catalyses the reaction of EC 1.14.13.197, dihydromonacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | monacolin J acid + [oxidized NADPH-hemoprotein reductase] + H2O | monacolin J acid i.e. (3R,5R)-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.124 | Aspergillus terreus | Q9Y7C8 | - |
- |
1.14.14.125 | Aspergillus terreus | Q9Y7C8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.124 | 3alpha-hydroxy-3,5-dihydromonacolin L acid | spontaneous | Aspergillus terreus | monacolin L acid + H2O | (1b), spontaneous | ? | |
1.14.14.124 | dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | Aspergillus terreus | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.124 | dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase | Aspergillus terreus | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.124 | dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.13.198, monacolin L hydroxylase | Aspergillus terreus | monacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.125 | monacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid. The enzyme also catalyses the reaction of EC 1.14.13.197, dihydromonacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | Aspergillus terreus | monacolin J acid + [oxidized NADPH-hemoprotein reductase] + H2O | monacolin J acid i.e. (3R,5R)-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid | ? | |
1.14.14.125 | monacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid. The enzyme also catalyses the reaction of EC 1.14.13.197, dihydromonacolin L hydroxylase | Aspergillus terreus | monacolin J acid + [oxidized NADPH-hemoprotein reductase] + H2O | monacolin J acid i.e. (3R,5R)-7-[(1S,2S,6R,8S,8aR)-8-hydroxy-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoic acid | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.124 | LovA | ambiguous | Aspergillus terreus |
1.14.14.125 | LovA | ambiguous | Aspergillus terreus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.124 | cytochrome P-450 | a heme-thiolate protein | Aspergillus terreus | |
1.14.14.125 | cytochrome P-450 | a heme-thiolate protein | Aspergillus terreus |