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Literature summary extracted from

  • Bullock, H.A.; Reisch, C.R.; Burns, A.S.; Moran, M.A.; Whitman, W.B.
    Regulatory and functional diversity of methylmercaptopropionate coenzyme A ligases from the dimethylsulfoniopropionate demethylation pathway in Ruegeria pomeroyi DSS-3 and other proteobacteria (2014), J. Bacteriol., 196, 1275-1285.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.44 expression in Escherichia coli Ruegeria pomeroyi
6.2.1.44 expression in Escherichia coli Candidatus Pelagibacter ubique
6.2.1.44 expression in Escherichia coli Ruegeria lacuscaerulensis
6.2.1.44 expression in Escherichia coli Pseudomonas aeruginosa
6.2.1.44 expression in Escherichia coli Burkholderia thailandensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.44 (NH4)2SO4 0.4 M, 93% inhibition Burkholderia thailandensis
6.2.1.44 (NH4)2SO4 0.4 M, 14% inhibition Candidatus Pelagibacter ubique
6.2.1.44 (NH4)2SO4 0.4 M, 29% inhibition; 0.4 M, 43% inhibition Ruegeria lacuscaerulensis
6.2.1.44 (NH4)2SO4 0.4 M, 75% inhibition Ruegeria pomeroyi
6.2.1.44 dimethylsulfoniopropionate inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge; inhibited by physiological concentrations (70 mM). ADP reverses the inhibition of RPO_DmdB1, suggesting that this enzyme was responsive to cellular energy charge. 3-(Methylthio)propanoate reverses the inhibition of RPO_DmdB2, suggesting that a complex regulatory system exists Ruegeria pomeroyi
6.2.1.44 K2SO4 0.4 M, 16% inhibition Candidatus Pelagibacter ubique
6.2.1.44 K2SO4 0.4 M, 5% inhibition; 0.4 M, 90% inhibition Ruegeria lacuscaerulensis
6.2.1.44 K2SO4 0.4 M, 68% inhibition Ruegeria pomeroyi
6.2.1.44 KCl 0.4 M, 73% inhibition Burkholderia thailandensis
6.2.1.44 KCl 0.4 M, 8% inhibition Candidatus Pelagibacter ubique
6.2.1.44 KCl 0.4 M, 55% inhibition; 0.4 M, 70% inhibition Ruegeria pomeroyi
6.2.1.44 Li2SO4 0.4 M, 92% inhibition Burkholderia thailandensis
6.2.1.44 Li2SO4 0.4 M, 92% inhibition Candidatus Pelagibacter ubique
6.2.1.44 Li2SO4 0.4 M, 41% inhibition; 0.4 M, 57% inhibition Ruegeria lacuscaerulensis
6.2.1.44 Li2SO4 0.4 M, 82% inhibition; 0.4 M, 82% inhibition Ruegeria pomeroyi
6.2.1.44 LiCl 0.4 M, 87% inhibition Burkholderia thailandensis
6.2.1.44 LiCl 0.4 M, 91% inhibition Candidatus Pelagibacter ubique
6.2.1.44 LiCl 0.4 M, 59% inhibition Ruegeria pomeroyi
6.2.1.44 additional information no inhibition by dimethylsulfoniopropionate Burkholderia thailandensis
6.2.1.44 additional information no inhibition by dimethylsulfoniopropionate Pseudomonas aeruginosa
6.2.1.44 additional information no inhibition by dimethylsulfoniopropionate; no inhibition by dimethylsulfoniopropionate Ruegeria lacuscaerulensis
6.2.1.44 Na2SO4 0.4 M, 84% inhibition Burkholderia thailandensis
6.2.1.44 Na2SO4 0.4 M, 60% inhibition Candidatus Pelagibacter ubique
6.2.1.44 Na2SO4 0.4 M, 58% inhibition; 0.4 M, 66% inhibition Ruegeria lacuscaerulensis
6.2.1.44 Na2SO4 0.4 M, 68% inhibition Ruegeria pomeroyi
6.2.1.44 NaCl 0.4 M, 92% inhibition Burkholderia thailandensis
6.2.1.44 NaCl 0.4 M, 57% inhibition Candidatus Pelagibacter ubique
6.2.1.44 NaCl 0.4 M,10% inhibition; 0.4 M, 14% inhibition Ruegeria lacuscaerulensis
6.2.1.44 NaCl 0.4 M, 50% inhibition; 0.4 M, 60% inhibition Ruegeria pomeroyi
6.2.1.44 NH4Cl 0.4 M, 55% inhibition Burkholderia thailandensis
6.2.1.44 NH4Cl 0.4 M, 32% inhibition Candidatus Pelagibacter ubique
6.2.1.44 NH4Cl 0.4 M, 50% inhibition Ruegeria lacuscaerulensis
6.2.1.44 NH4Cl 0.4 M, 94% inhibition Ruegeria pomeroyi
6.2.1.44 potassium acetate 0.4 M, 7% inhibition Burkholderia thailandensis
6.2.1.44 potassium acetate 0.4 M, 16% inhibition Candidatus Pelagibacter ubique
6.2.1.44 potassium acetate 0.4 M, 56% inhibition Ruegeria lacuscaerulensis
6.2.1.44 potassium acetate 0.4 M, 51% inhibition; 0.4 M, 73% inhibition Ruegeria pomeroyi
6.2.1.44 Sodium acetate 0.4 M, 45% inhibition Burkholderia thailandensis
6.2.1.44 Sodium acetate 0.4 M, 61% inhibition Candidatus Pelagibacter ubique
6.2.1.44 Sodium acetate 0.4 M, 68% inhibition; 0.4 M, 88% inhibition Ruegeria lacuscaerulensis
6.2.1.44 Sodium acetate 0.4 M, 66% inhibition; 0.4 M, 86% inhibition Ruegeria pomeroyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.44 0.01
-
Butyrate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 0.02
-
Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 0.04
-
propionate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 0.04
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 0.04
-
3-(methylthio)propanoate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 0.07
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 0.08
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 0.1 2 Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 0.44
-
Acrylate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 0.9
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 3.11
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 5.25
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.44 (NH4)2SO4 0.4 M, 1.5fold enhancement of activity Ruegeria pomeroyi
6.2.1.44 K2SO4 0.4 M, 1.3fold enhancement of activity Burkholderia thailandensis
6.2.1.44 K2SO4 0.4 M, 1.6fold enhancement of activity Ruegeria pomeroyi
6.2.1.44 KCl 0.4 M, 1.4fold enhancement of activity Ruegeria lacuscaerulensis
6.2.1.44 LiCl 0.4 M, 1.4fold enhancement of activity Ruegeria lacuscaerulensis
6.2.1.44 LiCl 0.4 M, 3fold enhancement of activity Ruegeria pomeroyi
6.2.1.44 Mg2+ required for activity Ruegeria pomeroyi
6.2.1.44 Mg2+ required for activity Candidatus Pelagibacter ubique
6.2.1.44 Mg2+ required for activity Ruegeria lacuscaerulensis
6.2.1.44 Mg2+ required for activity Pseudomonas aeruginosa
6.2.1.44 Mg2+ required for activity Burkholderia thailandensis
6.2.1.44 Na2SO4 0.4 M, 1.2fold enhancement of activity Ruegeria pomeroyi
6.2.1.44 NH4Cl 0.4 M, 1.6fold enhancement of activity Ruegeria lacuscaerulensis
6.2.1.44 NH4Cl 0.4 M, 2.1fold enhancement of activity Ruegeria pomeroyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.44 58000
-
2 * 58000, SDS-PAGE Ruegeria pomeroyi
6.2.1.44 59000
-
2 * 59000, SDS-PAGE Candidatus Pelagibacter ubique
6.2.1.44 59066
-
2 * 59066, calculated from sequence Ruegeria pomeroyi
6.2.1.44 59143
-
x * 59143, calculated from sequence Ruegeria lacuscaerulensis
6.2.1.44 59166
-
2 * 59166, calculated from sequence Ruegeria pomeroyi
6.2.1.44 59400
-
x * 59400, calculated from sequence Ruegeria lacuscaerulensis
6.2.1.44 59548
-
x * 59548, calculated from sequence Pseudomonas aeruginosa
6.2.1.44 61340
-
2 * 61340, calculated from sequence Candidatus Pelagibacter ubique
6.2.1.44 62000
-
2 * 62000, SDS-PAGE Ruegeria pomeroyi
6.2.1.44 64781
-
x * 64781, calculated from sequence Burkholderia thailandensis
6.2.1.44 108000
-
gel filtration Candidatus Pelagibacter ubique
6.2.1.44 112000
-
gel filtration Ruegeria pomeroyi
6.2.1.44 122000
-
gel filtration Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA Candidatus Pelagibacter ubique once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA Ruegeria pomeroyi once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA Ruegeria pomeroyi once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.44 Burkholderia thailandensis Q2SWN7
-
-
6.2.1.44 Burkholderia thailandensis ATCC 700388 Q2SWN7
-
-
6.2.1.44 Candidatus Pelagibacter ubique Q4FP19
-
-
6.2.1.44 Candidatus Pelagibacter ubique HTCC1062 Q4FP19
-
-
6.2.1.44 Pseudomonas aeruginosa Q9HWI3
-
-
6.2.1.44 Pseudomonas aeruginosa ATCC 15692 Q9HWI3
-
-
6.2.1.44 Ruegeria lacuscaerulensis D0CPY8
-
-
6.2.1.44 Ruegeria lacuscaerulensis D0CV95
-
-
6.2.1.44 Ruegeria lacuscaerulensis ITI-1157 D0CPY8
-
-
6.2.1.44 Ruegeria lacuscaerulensis ITI-1157 D0CV95
-
-
6.2.1.44 Ruegeria pomeroyi Q5LRT0
-
-
6.2.1.44 Ruegeria pomeroyi Q5LVM3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.44
-
Ruegeria pomeroyi
6.2.1.44
-
Candidatus Pelagibacter ubique
6.2.1.44
-
Ruegeria lacuscaerulensis
6.2.1.44
-
Pseudomonas aeruginosa
6.2.1.44
-
Burkholderia thailandensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.44 15
-
pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 16
-
pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 28
-
pH 7.5, 22°C Candidatus Pelagibacter ubique

Storage Stability

EC Number Storage Stability Organism
6.2.1.44 -20°C, stable in 100 mM HEPES for up to 6 months Candidatus Pelagibacter ubique
6.2.1.44 -20°C, stable in a solution of 100 mM KHPO4 for up to 2 months Ruegeria pomeroyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA
-
Ruegeria pomeroyi AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA
-
Candidatus Pelagibacter ubique AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA
-
Ruegeria lacuscaerulensis AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA
-
Pseudomonas aeruginosa AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA
-
Burkholderia thailandensis AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Candidatus Pelagibacter ubique AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi AMP + diphosphate + 3-(methylthio)propionyl-CoA
-
?
6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi AMP + diphosphate + 3-methylmercaptopropionyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 14% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 19% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 4% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 8% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + 3-methylbutanoyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 110% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 151% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 110% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acetate + CoA activity is 151% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acetyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 29% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 50% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 56% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 6% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 65% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 78% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 65% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 50% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 29% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + acrylate + CoA activity is 6% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acryloyl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 113% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 121% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 160% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 36% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 73% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 81% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 93% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 121% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 160% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 93% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 113% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + butyrate + CoA activity is 81% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + butyryl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 109% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 2% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 32% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 41% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 63% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 63% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + crotonate + CoA activity is 87% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + crotonyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 12% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 17% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 17% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 27% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 62% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + hexanoate + CoA activity is 94% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + hexanoyl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 12% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 30% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 48% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + isobutyrate + CoA activity is 7% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + isobutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 20% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 23% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 24% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 39% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + methylbutyrate + CoA activity is 8% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + methylbutyryl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 179% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 31% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 49% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 51% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 68% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 91% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 68% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + pentanoate + CoA activity is 179% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + pentanoyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 124% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 126% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 16% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 45% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 72% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 77% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 124% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 45% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 72% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 ATP + propionate + CoA activity is 126% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + propionyl-CoA
-
?
6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria pomeroyi ?
-
?
6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria lacuscaerulensis ?
-
?
6.2.1.44 additional information no activity with acetate, caprylate and caprate Burkholderia thailandensis ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Ruegeria pomeroyi ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Candidatus Pelagibacter ubique ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Ruegeria lacuscaerulensis ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Pseudomonas aeruginosa ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Pseudomonas aeruginosa ATCC 15692 ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Candidatus Pelagibacter ubique HTCC1062 ?
-
?
6.2.1.44 additional information no activity with acetate, caprylate and caprate Burkholderia thailandensis ATCC 700388 ?
-
?
6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria lacuscaerulensis ITI-1157 ?
-
?
6.2.1.44 additional information no activity with caprylate and caprate Ruegeria lacuscaerulensis ITI-1157 ?
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.44 ? x * 59143, calculated from sequence Ruegeria lacuscaerulensis
6.2.1.44 ? x * 59400, calculated from sequence Ruegeria lacuscaerulensis
6.2.1.44 ? x * 59548, calculated from sequence Pseudomonas aeruginosa
6.2.1.44 ? x * 64781, calculated from sequence Burkholderia thailandensis
6.2.1.44 dimer 2 * 58000, SDS-PAGE Ruegeria pomeroyi
6.2.1.44 dimer 2 * 62000, SDS-PAGE Ruegeria pomeroyi
6.2.1.44 dimer 2 * 59000, SDS-PAGE Candidatus Pelagibacter ubique
6.2.1.44 dimer 2 * 59066, calculated from sequence Ruegeria pomeroyi
6.2.1.44 dimer 2 * 59166, calculated from sequence Ruegeria pomeroyi
6.2.1.44 dimer 2 * 61340, calculated from sequence Candidatus Pelagibacter ubique

Synonyms

EC Number Synonyms Comment Organism
6.2.1.44 BTH_DmdB2
-
Burkholderia thailandensis
6.2.1.44 BTH_I2141
-
Burkholderia thailandensis
6.2.1.44 DmdB
-
Candidatus Pelagibacter ubique
6.2.1.44 DmdB
-
Ruegeria lacuscaerulensis
6.2.1.44 DmdB
-
Pseudomonas aeruginosa
6.2.1.44 DmdB
-
Burkholderia thailandensis
6.2.1.44 DmdB two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi
6.2.1.44 methylmercaptopropionate-coenzyme A ligase
-
Ruegeria pomeroyi
6.2.1.44 methylmercaptopropionate-coenzyme A ligase
-
Candidatus Pelagibacter ubique
6.2.1.44 methylmercaptopropionate-coenzyme A ligase
-
Ruegeria lacuscaerulensis
6.2.1.44 methylmercaptopropionate-coenzyme A ligase
-
Pseudomonas aeruginosa
6.2.1.44 methylmercaptopropionate-coenzyme A ligase
-
Burkholderia thailandensis
6.2.1.44 MMPA-CoA ligase
-
Ruegeria pomeroyi
6.2.1.44 MMPA-CoA ligase
-
Candidatus Pelagibacter ubique
6.2.1.44 MMPA-CoA ligase
-
Ruegeria lacuscaerulensis
6.2.1.44 MMPA-CoA ligase
-
Pseudomonas aeruginosa
6.2.1.44 MMPA-CoA ligase
-
Burkholderia thailandensis
6.2.1.44 PA4198
-
Pseudomonas aeruginosa
6.2.1.44 PA_DmdB1
-
Pseudomonas aeruginosa
6.2.1.44 PU_DmdB1
-
Candidatus Pelagibacter ubique
6.2.1.44 RL_DmdB1
-
Ruegeria lacuscaerulensis
6.2.1.44 RL_DmdB2
-
Ruegeria lacuscaerulensis
6.2.1.44 RPO_DmdB1 two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi
6.2.1.44 RPO_DmdB2 two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi
6.2.1.44 SAR11_0248
-
Candidatus Pelagibacter ubique
6.2.1.44 SL1157_1815
-
Ruegeria lacuscaerulensis
6.2.1.44 SL1157_2728
-
Ruegeria lacuscaerulensis
6.2.1.44 SPO0677
-
Ruegeria pomeroyi
6.2.1.44 SPO2045
-
Ruegeria pomeroyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.44 22
-
assay at room temperature Ruegeria pomeroyi
6.2.1.44 22
-
assay at room temperature Candidatus Pelagibacter ubique

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.44 10 30 activity at 10°C and at 30°C is nearly the same as at room temperature Ruegeria pomeroyi
6.2.1.44 10 30 activity at 10°C and at 30°C is nearly the same as at room temperature Candidatus Pelagibacter ubique

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.44 1
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 3.7
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 7.2
-
Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 10.8
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 14.3
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 14.4
-
Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 14.9
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 17.7
-
propionate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 18.7
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 22.3
-
Acrylate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 29.4
-
3-(methylthio)propanoate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 43.8
-
Butyrate pH 7.5, 22°C Candidatus Pelagibacter ubique

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.44 7
-
-
Ruegeria pomeroyi
6.2.1.44 7.5
-
-
Ruegeria pomeroyi
6.2.1.44 7.5
-
-
Candidatus Pelagibacter ubique

Expression

EC Number Organism Comment Expression
6.2.1.44 Ruegeria pomeroyi two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on dimethylsulfoniopropionate and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation additional information

General Information

EC Number General Information Comment Organism
6.2.1.44 metabolism two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2. They are differentially expressed depending on carbon source. RPO_dmdB1 exhibits overall lower steady-state levels of mRNA than RPO_dmdB2 for dimethylsulfoniopropionate, 3-(methylthio)propanoate, or methionine as the sole source of carbon. Although the levels of the RPO_dmdB1 transcripts increases during growth on DMSP and methionine, it is always lower than those of RPO_dmdB2. For that reason, RPO_dmdB2 appears to be the major DmdB during growth on compounds leading to 3-(methylthio)propanoate formation Ruegeria pomeroyi

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.44 0.2
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 1.2
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 16
-
Acrylate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 50
-
Acrylate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 71
-
Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 213
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 233
-
3-(methylthio)propanoate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 271
-
propionate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 505
-
propionate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 735
-
3-(methylthio)propanoate pH 7.5, 22°C Candidatus Pelagibacter ubique
6.2.1.44 1031
-
Butyrate pH 7.5, 22°C Ruegeria pomeroyi
6.2.1.44 3370
-
Butyrate pH 7.5, 22°C Candidatus Pelagibacter ubique