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Literature summary extracted from

  • Xing, X.; Zhang, L.; Guo, L.; She, Q.; Huang, L.
    Sulfolobus replication factor C stimulates the activity of DNA polymerase B1 (2014), J. Bacteriol., 196, 2367-2375.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 Replication factor C enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.B8 recombinant expression of small subunit RFCS, and full-length enzyme RFCSL in Escherichia coli strain Rosetta(DE3)/pLysS Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.4.B8 R84A/R90A/T120A/K149A site-directed mutagenesis, quadruple mutant of RFCS small subunit Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.B8 Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.B8 ATP + H2O Saccharolobus solfataricus
-
 ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O Saccharolobus solfataricus P2
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus
-
-
-
3.6.4.B8 Saccharolobus solfataricus Q9UXF5 small subunit
-
3.6.4.B8 Saccharolobus solfataricus Q9UXF6 large subunit
-
3.6.4.B8 Saccharolobus solfataricus P2 Q9UXF5 small subunit
-
3.6.4.B8 Saccharolobus solfataricus P2 Q9UXF6 large subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.B8 ATP + H2O
-
 Saccharolobus solfataricus ADP + phosphate
-
 ?
3.6.4.B8 ATP + H2O
-
 Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.4.B8 heteropentamer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 POlB1
-
 Saccharolobus solfataricus
3.6.4.B8 RFC
-
 Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.6.4.B8 physiological function RFC is an ATPase. The RFC complex from Sulfolobus solfataricus physically interacts with DNA polymerase B1 (PolB1) and enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner. Stimulation of the PolB1 activity by RFC is independent of the ability of RFC to bind DNA but is consistent with the ability of RFC to facilitate DNA binding by PolB1 through protein-protein interaction. Sulfolobus RFC may play a role in recruiting DNA polymerase for efficient primer extension, in addition to clamp loading, during DNA replication. RFC is shown to interact with the PCNA1 and PCNA2 subunits through its small subunit RFCS and with PCNA3 through its large subunit RFCL Saccharolobus solfataricus