Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jones, J.B.; Stadtman, T.C.
    Selenium-dependent and selenium-independent formate dehydrogenases of Methanococcus vannielii. Separation of the two forms and characterization of the purified selenium-independent form (1981), J. Biol. Chem., 256, 656-663.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.98.4 1,10-phenanthroline
-
 Methanococcus vannielii
1.17.98.4 1,2-dihydroxybenzene-3,5-disulfonic acid
-
 Methanococcus vannielii
1.17.98.4 2,2'-dipyridyl
-
 Methanococcus vannielii
1.17.98.4 4,5-dihydroxybenzene-1,3-disulfonate
-
 Methanococcus vannielii
1.17.98.4 EDTA
-
 Methanococcus vannielii
1.17.98.4 iodoacetamide addition of 1 mM iodoacetamide, to enzyme preparations in pH 7 buffer containing 2 mM 2-mercaptoethanol inactivates Methanococcus vannielii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.98.4 selenium anaerobic oxidation of formate by Methanococcus vannielii is catalyzed by two readily separable formate dehydrogenases. One of these is a 105000 Da protein that contains molybdenum, iron, and acid-labile sulfide, but not selenium. The other is a high molecular weight complex composed of selenoprotein and molybdo-iron sulfur protein subunits Methanococcus vannielii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.98.4 105000
-
 anaerobic oxidation of formate by Methanococcus vannielii is catalyzed by two readily separable formate dehydrogenases. One of these is a 105000 Da protein that contains molybdenum, iron, and acid-labile sulfide, but not selenium. The other is a high molecular weight complex composed of selenoprotein and molybdo-iron sulfur protein subunits Methanococcus vannielii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.98.4 formate + oxidized coenzyme F420 Methanococcus vannielii
-
 CO2 + reduced coenzyme F420
-
 ?
1.17.98.4 formate + oxidized coenzyme F420 Methanococcus vannielii DSM 1224
-
 CO2 + reduced coenzyme F420
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.98.4 Methanococcus vannielii
-
-
-
1.17.98.4 Methanococcus vannielii DSM 1224
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.98.4 extremely oxygen-sensitive Methanococcus vannielii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.98.4 formate + FAD
-
 Methanococcus vannielii CO2 + FADH2
-
 ?
1.17.98.4 formate + FAD
-
 Methanococcus vannielii DSM 1224 CO2 + FADH2
-
 ?
1.17.98.4 formate + FMN
-
 Methanococcus vannielii CO2 + FMNH2
-
 ?
1.17.98.4 formate + FMN
-
 Methanococcus vannielii DSM 1224 CO2 + FMNH2
-
 ?
1.17.98.4 formate + oxidized coenzyme F420
-
 Methanococcus vannielii CO2 + reduced coenzyme F420
-
 ?
1.17.98.4 formate + oxidized coenzyme F420
-
 Methanococcus vannielii DSM 1224 CO2 + reduced coenzyme F420
-
 ?
1.17.98.4 additional information no activity with NADP+ or NAD+ Methanococcus vannielii ?
-
 ?
1.17.98.4 additional information no activity with NADP+ or NAD+ Methanococcus vannielii DSM 1224 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.98.4 60
-
-
 Methanococcus vannielii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.98.4 8.5 9.2
-
 Methanococcus vannielii