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Literature summary extracted from

  • Dejima, K.; Takemura, M.; Nakato, E.; Peterson, J.; Hayashi, Y.; Kinoshita-Toyoda, A.; Toyoda, H.; Nakato, H.
    Analysis of Drosophila glucuronyl C5-epimerase: implications for developmental roles of heparan sulfate sulfation compensation and 2-O-sulfated glucuronic acid (2013), J. Biol. Chem., 288, 34384-34393.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.17 additional information construction of enzyme deficient strains ussing P-element transposase exscision mutagenesis, removal of the entire Hsepi coding sequence, generation of viable and fertile homozygous mutants for Hsepid12 and Hsepid13 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate Drosophila melanogaster
-
 Heparosan-N-sulfate L-iduronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.17 Drosophila melanogaster
-
 gene Hsepi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.17 Heparosan-N-sulfate D-glucuronate
-
 Drosophila melanogaster Heparosan-N-sulfate L-iduronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.1.3.17 glucuronyl C5-epimerase
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
5.1.3.17 malfunction homozygous enzyme mutant flies are viable and fertile with only minor morphological defects, including the formation of an ectopic crossvein in the wing, but they have a short lifespan. Loss of Hsepi results in a significant impairment of 2-O-sulfation and induces compensatory increases in N- and 6-O-sulfation. Simultaneous block of Hsepi glucuronyl C5-epimerase and heparan sulfate 6-O-sulfotransferase activity disrupted tracheoblast formation, a well established FGF-dependent process. The increase in 6-O-sulfation in Hsepi mutants is critical for the rescue of FGF signaling Drosophila melanogaster
5.1.3.17 additional information disaccharide analyses of chondroitin sulfate from enzyme wild-type and mutant strains, with or without additional mutation of heparan sulfate 2-O-sulfotransferase, overview Drosophila melanogaster
5.1.3.17 physiological function during the biosynthesis of heparan sulfate, glucuronyl C5-epimerase catalyzes C5-epimerization of glucuronic acid, converting it to iduronic acid. C5-epimerization is required for normal heparan sulfate sulfation. Role of Hsepi in development, overview. Genetic interactions between the enzyme and HSPG core protein genes during wing margin formation Drosophila melanogaster