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BRENDA support

Literature summary extracted from

  • Tural, B.; Tarhan, T.; Tural, S.
    Covalent immobilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic epoxy support and its carboligation reactivity (2014), J. Mol. Catal. B, 102, 188-194.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 recombinant expression of His-tagged enzyme in Escherichia coli strain SG13009 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 additional information covalent immobilization and stabilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic nanoparticles by a three-step immobilization/stabilization procedure at pH 8.0, 20°C, method development and evaluation, overview. The maximum enzyme loading is found to be 5.2 mg/g support in 3.5 mg/ml initial enzyme concentration at ionic strength of 1.25 M MgSO4. The covalently-bound enzyme is characterized in terms of its activity and stability for the formation of (S)-2-hydroxypropiophenone. The immobilized biocatalyst retains 95% of its original activity after five reaction cycles Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.7 Mg2+ required Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 recombinant His-tagged enzyme from Escherichia coli strain SG13009 by nickel affinity chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 additional information synthesis of (S)-2-hydroxy-1-phenylpropanone by the immobilized recombinant enzyme is effective in cross acyloin reaction of benzaldehyde with acetaldehyde. The immobilized and free enzymes shows similar substrate specificity and activity Pseudomonas putida ?
-
?
4.1.1.7 phenylglyoxylate
-
Pseudomonas putida benzaldehyde + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFD
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.7 20
-
assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.7 7.5
-
assay at, substrate phenylglyoxylate Pseudomonas putida
4.1.1.7 8.5
-
assay at, cross acyloin reaction of benzaldehyde with acetaldehyde Pseudomonas putida