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Literature summary extracted from

  • Torres, P.; Manzo, R.; Rubiolo, A.; Batista-Viera, F.; Mammarella, E.
    Purification of an L-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy (2014), J. Mol. Catal. B, 102, 99-105.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 L-arabitol competitive inhibitor Enterococcus faecium
5.3.1.4 L-ribitol
-
 Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 3 5 D-galactose at pH 7.0 and 50°C Enterococcus faecium
5.3.1.4 42
-
 L-arabinose at pH 7.0 and 50°C Enterococcus faecium
5.3.1.4 71
-
 D-galactose at pH 7.0 and 40°C Enterococcus faecium
5.3.1.4 101
-
 D-galactose at pH 7.0 and 30°C Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 48000
-
 2 * 48000, SDS-PAGE Enterococcus faecium
5.3.1.4 187000
-
 gel filtration Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.4 L-arabinose Enterococcus faecium
-
 L-ribulose
-
 ?
5.3.1.4 L-arabinose Enterococcus faecium DBFIQ E36
-
 L-ribulose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Enterococcus faecium
-
-
-
5.3.1.4 Enterococcus faecium DBFIQ E36
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4 ammonium sulfate precipitation, epoxy-activated Sepharose column chromatography, and L-arabitol-agarose column chromatography Enterococcus faecium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 0.25
-
 cell-free extract, at pH 7.0 and 50°C Enterococcus faecium
5.3.1.4 180
-
 after 720fold purification, at pH 7.0 and 50°C Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-galactose
-
 Enterococcus faecium D-tagatose
-
 ?
5.3.1.4 D-galactose
-
 Enterococcus faecium DBFIQ E36 D-tagatose
-
 ?
5.3.1.4 L-arabinose
-
 Enterococcus faecium L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Enterococcus faecium DBFIQ E36 L-ribulose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 homotetramer 2 * 48000, SDS-PAGE Enterococcus faecium

Synonyms

EC Number Synonyms Comment Organism
5.3.1.4 L-AI
-
 Enterococcus faecium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.4 50
-
 activity declines over 60°C Enterococcus faecium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.4 45 70 the enzyme possesses good stability at 45-50°C, with half-life of 10 h at 50°C. The stability strongly decreases in the range 55-70°C Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 7
-
 70% of maximal activity at pH 4.5 Enterococcus faecium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.4 6.5 8.5 betweem pH 6.0 and 8.5, the enzyme remains stable with maximum stability at pH 7.0-7.5. The stability decreases moderately at alkaline pH. Enzyme retains more than 60% of its activity when incubated 6 h at pH 6.5-8.5 Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.4 7.9
-
 L-arabitol at pH 7.0 and 50°C Enterococcus faecium
5.3.1.4 183
-
 L-ribitol at pH 7.0 and 50°C Enterococcus faecium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.4 Enterococcus faecium isoelectric focusing
-
 3.8