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Literature summary extracted from

  • Ito, H.; Tanaka, A.
    Evolution of a new chlorophyll metabolic pathway driven by the dynamic changes in enzyme promiscuous activity (2014), Plant Cell Physiol., 55, 593-603.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.2 expressed in Escherichia coli BL-21 as a His-tagged fusion protein Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.13 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster Synechocystis sp.
-
 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.13 Synechocystis sp. P74473
-
-
1.17.7.2 Synechocystis sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.2 using Ni-NTA chromatography Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.13 protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster
-
 Synechocystis sp. 3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.13 3,8-divinyl chlorophyllide reductase
-
 Synechocystis sp.
1.3.7.13 bciB
-
 Synechocystis sp.
1.3.7.13 F-DVR
-
 Synechocystis sp.
1.3.7.13 Slr1923
-
 Synechocystis sp.
1.17.7.2 3,8-divinyl chlorophyllide reductase
-
 Synechocystis sp.
1.17.7.2 DVR
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.7.2 25
-
 assay at Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.7.2 7.5
-
 assay at Synechocystis sp.

General Information

EC Number General Information Comment Organism
1.17.7.2 metabolism cyanobacterial 3,8-divinyl chlorophyllide reductase (DVR) has 7-hydroxymethyl chlorophyll a reductase activity. Enzyme participates in the Chl cycle in green plants Synechocystis sp.