BRENDA - Enzyme Database show

A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ-dependent manner in Catharanthus roseus

Besseau, S.; Kellner, F.; Lanoue, A.; Thamm, A.M.; Salim, V.; Schneider, B.; Geu-Flores, F.; Hoefer, R.; Guirimand, G.; Guihur, A.; Oudin, A.; Glevarec, G.; Foureau, E.; Papon, N.; Clastre, M.; Giglioli-Guivarch, N.; St-Pierre, B.; Werck-Reichhart, D.; Burlat, V.; De Luca, V.; OConnor, S.E.; Cou, C.o.u.r.; Plant Physiol. 163, 1792-1803 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.103
expressed in Saccharomyces cerevisiae WAT11 cells
Catharanthus roseus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.103
0.00007
-
tabersonine
isoform T16H2, at pH 8.0 and 30°C
Catharanthus roseus
1.14.14.103
0.00035
-
tabersonine
isoform T16H1, at pH 8.0 and 30°C
Catharanthus roseus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.103
endoplasmic reticulum
CYP71D351 (isoform T16H2)
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.103
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.103
additional information
Catharanthus roseus
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
highest activity
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.103
Catharanthus roseus
C0KYN4
CYP71D12; cultivar Pacifica Pink
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
flower
isoform T16H1, CYP71D351
Catharanthus roseus
-
1.14.14.103
leaf epidermis
highest expression of isoform T16H2, CYP71D12 in young leaves
Catharanthus roseus
-
1.14.14.103
additional information
CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds; CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds
Catharanthus roseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.103
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.103
additional information
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
728525
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
highest activity
728525
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.103
expressed in Saccharomyces cerevisiae WAT11 cells
Catharanthus roseus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.103
0.00007
-
tabersonine
isoform T16H2, at pH 8.0 and 30°C
Catharanthus roseus
1.14.14.103
0.00035
-
tabersonine
isoform T16H1, at pH 8.0 and 30°C
Catharanthus roseus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.103
endoplasmic reticulum
CYP71D351 (isoform T16H2)
Catharanthus roseus
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.103
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.103
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
Catharanthus roseus
low activity
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.103
additional information
Catharanthus roseus
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
?
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
Catharanthus roseus
highest activity
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.103
flower
isoform T16H1, CYP71D351
Catharanthus roseus
-
1.14.14.103
leaf epidermis
highest expression of isoform T16H2, CYP71D12 in young leaves
Catharanthus roseus
-
1.14.14.103
additional information
CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds; CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.103
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.103
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2
low activity
728525
Catharanthus roseus
2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.103
additional information
no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine
728525
Catharanthus roseus
?
-
-
-
-
1.14.14.103
tabersonine + NADPH + H+ + O2
highest activity
728525
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.14.103
metabolism
CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers; CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves
Catharanthus roseus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.103
metabolism
CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers; CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves
Catharanthus roseus