EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.93 | recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts | Arabidopsis thaliana |
4.2.1.93 | recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts | Zea mays |
4.2.1.136 | transient expression of GFP-tagged enzyme in Nicotiana tabacum BY-2 cells, expression of N-terminally His-tagged mature dehydratase in Escherichia coli strain BL21 (DE3) RIPL | Arabidopsis thaliana |
5.1.99.6 | expression of N-terminally His-tagged enzyme in Escherichia coli | Zea mays |
5.1.99.6 | expression of N-terminally His-tagged enzyme in Escherichia coli | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.136 | additional information | construction of enzyme knockout lines using T-DNA insertion, phenotype, overview | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.93 | 0.00069 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.93 | 0.00086 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.93 | 0.00089 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
4.2.1.93 | 0.00115 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 0.00069 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 0.00086 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 0.00089 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 0.00115 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
5.1.99.6 | 0.0006 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH and temperature not specified in the publication | Arabidopsis thaliana | |
5.1.99.6 | 0.00115 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH and temperature not specified in the publication | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.93 | chloroplast | import assays with purified pea (Pisum sativum) chloroplasts and mitochondria | Arabidopsis thaliana | 9507 | - |
4.2.1.93 | chloroplast | import assays with purified pea (Pisum sativum) chloroplasts and mitochondria | Zea mays | 9507 | - |
4.2.1.93 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
4.2.1.93 | cytosol | - |
Zea mays | 5829 | - |
4.2.1.93 | mitochondrion | import assays with purified pea (Pisum sativum) chloroplasts and mitochondria | Arabidopsis thaliana | 5739 | - |
4.2.1.93 | mitochondrion | import assays with purified pea (Pisum sativum) chloroplasts and mitochondria | Zea mays | 5739 | - |
4.2.1.136 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
4.2.1.136 | chloroplast | - |
Zea mays | 9507 | - |
4.2.1.136 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
4.2.1.136 | cytosol | - |
Zea mays | 5829 | - |
4.2.1.136 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
4.2.1.136 | mitochondrion | - |
Zea mays | 5739 | - |
4.2.1.136 | additional information | the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX | Arabidopsis thaliana | - |
- |
4.2.1.136 | additional information | the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX | Zea mays | - |
- |
5.1.99.6 | chloroplast | - |
Zea mays | 9507 | - |
5.1.99.6 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
5.1.99.6 | cytoplasm | - |
Zea mays | 5737 | - |
5.1.99.6 | cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
5.1.99.6 | mitochondrion | - |
Zea mays | 5739 | - |
5.1.99.6 | mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.136 | Mg2+ | required | Arabidopsis thaliana | |
4.2.1.136 | Mg2+ | required | Zea mays |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Zea mays | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Col-0 | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Columbia-0 | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Zea mays | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Col-0 | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Columbia-0 | stereospecific for (S)-NAD(P)HX epimer | ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana | - |
ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Zea mays | - |
ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana Columbia-0 | - |
ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana | - |
ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Zea mays | - |
ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Col-0 | - |
ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana Columbia-0 | - |
ADP + phosphate + NADPH | - |
? | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Zea mays | i.e. NADHX | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | Arabidopsis thaliana | i.e. NADHX | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Zea mays | i.e. NADPHX | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | Arabidopsis thaliana | i.e. NADPHX | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.93 | Arabidopsis thaliana | Q94AF2 | gene At5g19150 | - |
4.2.1.93 | Arabidopsis thaliana Col-0 | Q94AF2 | gene At5g19150 | - |
4.2.1.93 | Zea mays | A0A3L6F337 | - |
- |
4.2.1.136 | Arabidopsis thaliana | Q94AF2 | - |
- |
4.2.1.136 | Arabidopsis thaliana Columbia-0 | Q94AF2 | - |
- |
4.2.1.136 | Zea mays | A0A3L6F337 | - |
- |
5.1.99.6 | Arabidopsis thaliana | Q9LTX3 | - |
- |
5.1.99.6 | Zea mays | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.136 | recombinant N-terminally His-tagged mature dehydratase from Escherichia coli strain BL21 (DE3) RIPL by nickel affinity chromatography | Arabidopsis thaliana |
5.1.99.6 | recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Zea mays |
5.1.99.6 | recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.93 | seedling | - |
Arabidopsis thaliana | - |
4.2.1.136 | leaf | - |
Arabidopsis thaliana | - |
4.2.1.136 | leaf | - |
Zea mays | - |
4.2.1.136 | seedling | - |
Arabidopsis thaliana | - |
5.1.99.6 | seedling | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Zea mays | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Col-0 | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Zea mays | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Col-0 | ADP + phosphate + NADPH | - |
? | |
4.2.1.93 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | stereospecific for (S)-NAD(P)HX epimer | Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana | ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Zea mays | ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana | ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Zea mays | ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana Col-0 | ADP + phosphate + NADPH | - |
? | |
4.2.1.136 | ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
Arabidopsis thaliana Columbia-0 | ADP + phosphate + NADPH | - |
? | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | i.e. NADHX | Zea mays | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | i.e. NADHX | Arabidopsis thaliana | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | i.e. NADPHX | Zea mays | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
r | |
5.1.99.6 | (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | i.e. NADPHX | Arabidopsis thaliana | (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.93 | (S)-NAD(P)HX dehydratase | - |
Arabidopsis thaliana |
4.2.1.93 | (S)-NAD(P)HX dehydratase | - |
Zea mays |
4.2.1.136 | NAD(P)HX dehydratase | - |
Arabidopsis thaliana |
4.2.1.136 | NAD(P)HX dehydratase | - |
Zea mays |
5.1.99.6 | epimerase-PPOX | - |
Zea mays |
5.1.99.6 | epimerase-PPOX | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.93 | 22 | - |
assay at | Arabidopsis thaliana |
4.2.1.93 | 22 | - |
assay at | Zea mays |
4.2.1.136 | 22 | - |
assay at | Arabidopsis thaliana |
4.2.1.136 | 22 | - |
assay at | Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.93 | 0.26 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.93 | 0.3 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.93 | 0.34 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.93 | 0.38 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 0.26 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 0.3 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 0.34 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 0.38 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
5.1.99.6 | 0.26 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH and temperature not specified in the publication | Arabidopsis thaliana | |
5.1.99.6 | 0.38 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH and temperature not specified in the publication | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.93 | 8 | - |
assay at | Arabidopsis thaliana |
4.2.1.93 | 8 | - |
assay at | Zea mays |
4.2.1.136 | 8 | - |
assay at | Arabidopsis thaliana |
4.2.1.136 | 8 | - |
assay at | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.93 | ATP | dependent on | Arabidopsis thaliana | |
4.2.1.93 | ATP | dependent on | Zea mays | |
4.2.1.136 | ADP | dependent on | Arabidopsis thaliana | |
4.2.1.136 | ADP | dependent on | Zea mays | |
5.1.99.6 | additional information | the hydratase is dependent on ATP, but the epimerase ist not | Zea mays | |
5.1.99.6 | additional information | the hydratase is dependent on ATP, but the epimerase ist not | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.93 | evolution | domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants | Arabidopsis thaliana |
4.2.1.93 | evolution | domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants | Zea mays |
4.2.1.93 | physiological function | the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Arabidopsis thaliana |
4.2.1.93 | physiological function | the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Zea mays |
4.2.1.136 | malfunction | ablation of the Arabidopsis dehydratase gene raises seedling levels of all NADHX forms by 20 to 40fold, and levels of one NADPHX form by 10 to 30fold | Arabidopsis thaliana |
4.2.1.136 | metabolism | plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Arabidopsis thaliana |
4.2.1.136 | metabolism | plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites | Zea mays |
4.2.1.136 | physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner | Arabidopsis thaliana |
4.2.1.136 | physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner | Zea mays |
5.1.99.6 | malfunction | a truncated form of the Arabidopsis epimerase lacking the PPOX domain is also fully active in the interconversion | Arabidopsis thaliana |
5.1.99.6 | additional information | the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family | Zea mays |
5.1.99.6 | additional information | the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family | Arabidopsis thaliana |
5.1.99.6 | physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments | Zea mays |
5.1.99.6 | physiological function | NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.93 | 262 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.93 | 358 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.93 | 509 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.93 | 524 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 262 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 358 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide | pH 8.0, 22°C | Zea mays | |
4.2.1.136 | 509 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Arabidopsis thaliana | |
4.2.1.136 | 524 | - |
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate | pH 8.0, 22°C | Zea mays |