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Literature summary extracted from

  • Tondo, M.L.; Musumeci, M.A.; Delprato, M.L.; Ceccarelli, E.A.; Orellano, E.G.
    Structural-functional characterization and physiological significance of ferredoxin-NADP reductase from Xanthomonas axonopodis pv. citri (2011), PLoS ONE, 6, e27124.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expression in an enzyme knock-out Eschwerichia coli strain Xanthomonas axonopodis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0108
-
 NADPH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis
1.18.1.2 3.3
-
 NADH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NADP+ Xanthomonas axonopodis ferredoxin Xac1762 is a potential substrate 2 oxidized ferredoxin + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Xanthomonas axonopodis Q8PMH0 pv. citri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NAD+ NAD+ is a poor substrate Xanthomonas axonopodis 2 oxidized ferredoxin + NADH + H+
-
 ?
1.18.1.2 2 reduced ferredoxin + NADP+ ferredoxin Xac1762 is a potential substrate Xanthomonas axonopodis 2 oxidized ferredoxin + NADPH + H+
-
 ?
1.18.1.2 additional information no substrates: pea ferredoxin, Escherichia coli ferredoxin, flavodoxin Xanthomonas axonopodis ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.18.1.2 48.1
-
 melting temperature Xanthomonas axonopodis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 3.2
-
 NADH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis
1.18.1.2 121.9
-
 NADPH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 NADH specificity NADPH/NADH is 1600 Xanthomonas axonopodis
1.18.1.2 NADPH specificity NADPH/NADH is 1600 Xanthomonas axonopodis

Expression

EC Number Organism Comment Expression
1.18.1.2 Xanthomonas axonopodis a 2.2fold induction of expression is observed in the presence of the superoxide-generating agents methyl viologen and 2,3-dimethoxy-1,4-naphthoquinone up

General Information

EC Number General Information Comment Organism
1.18.1.2 physiological function enzyme is able to substitute for the ferredoxin-NADP+-reductase in Escherichia coli in its antioxidant role. It is involved in the oxidative stress response of Xanthomonas axonopodis pv. citri and performs its biological function most likely through the interaction with ferredoxin XAC1762 Xanthomonas axonopodis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 1
-
 NADH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis
1.18.1.2 11300
-
 NADPH pH 8.0, temperature not specified in the publication Xanthomonas axonopodis