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Literature summary extracted from

  • Inouye, S.; Sahara-Miura, Y.; Sato, J.; Iimori, R.; Yoshida, S.; Hosoya, T.
    Expression, purification and luminescence properties of coelenterazine-utilizing luciferases from Renilla, Oplophorus and Gaussia: comparison of substrate specificity for C2-modified coelenterazines (2013), Protein Expr. Purif., 88, 150-156.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.5 catalytic domain (19 kDa) is expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Oplophorus gracilirostris
1.13.12.5 expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Renilla reniformis
1.13.12.5 expressed using a cold-induced expression system in Escherichia coli as His-tagged fusion proteins Gaussia
1.13.12.13 the catalytic domain of Oplophorus luciferase (19kOLase) is expressed in Escherichia coli cells using the cold-induced vectors of pCold-KAZ. Cold induction for Escherichia coli cells is carried out at 15°C Oplophorus gracilirostris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.5 19000
-
 catalytic domain Oplophorus gracilirostris
1.13.12.5 37600
-
 SDS-PAGE Renilla reniformis
1.13.12.5 37700
-
 calculated from sequence Renilla reniformis
1.13.12.5 38040
-
 calculated from sequence, 4red-shift luciferase Renilla reniformis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.5 Gaussia
-
-
-
1.13.12.5 Oplophorus gracilirostris
-
-
-
1.13.12.5 Renilla reniformis
-
-
-
1.13.12.13 Oplophorus gracilirostris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.5 using Ni-chelate column Renilla reniformis
1.13.12.5 using Ni-chelate column Oplophorus gracilirostris
1.13.12.5 using Ni-chelate column Gaussia
1.13.12.13 using nickel-chelate affinity column Oplophorus gracilirostris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.5 3iso-coelenterazine + O2 relative activity to native coelenterazine: 11.6% Renilla reniformis ?
-
 ?
1.13.12.5 3iso-coelenterazine + O2 relative activity to native coelenterazine: 14.3% Gaussia ?
-
 ?
1.13.12.5 3iso-coelenterazine + O2 relative activity to native coelenterazine: 34.9% Renilla reniformis ?
-
 ?
1.13.12.5 3iso-coelenterazine + O2 relative activity to native coelenterazine: 78.2% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 3me-coelenterazine + O2 relative activity to native coelenterazine: 11.8% Renilla reniformis ?
-
 ?
1.13.12.5 3me-coelenterazine + O2 relative activity to native coelenterazine: 2.6% Gaussia ?
-
 ?
1.13.12.5 3me-coelenterazine + O2 relative activity to native coelenterazine: 45.5% Renilla reniformis ?
-
 ?
1.13.12.5 3me-coelenterazine + O2 relative activity to native coelenterazine: 80% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 3meo-coelenterazine + O2 relative activity to native coelenterazine: 189% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 3meo-coelenterazine + O2 relative activity to native coelenterazine: 3.8% Gaussia ?
-
 ?
1.13.12.5 3meo-coelenterazine + O2 relative activity to native coelenterazine: 5.5% Renilla reniformis ?
-
 ?
1.13.12.5 3meo-coelenterazine + O2 relative activity to native coelenterazine: 82.3% Renilla reniformis ?
-
 ?
1.13.12.5 alphameh-coelenterazine + O2 relative activity to native coelenterazine: 0.02% Renilla reniformis ?
-
 ?
1.13.12.5 alphameh-coelenterazine + O2 relative activity to native coelenterazine: 0.02% Gaussia ?
-
 ?
1.13.12.5 alphameh-coelenterazine + O2 relative activity to native coelenterazine: 15.7% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 alphameh-coelenterazine + O2 relative activity to native coelenterazine: 24.7% Renilla reniformis ?
-
 ?
1.13.12.5 cf3-coelenterazine + O2 relative activity to native coelenterazine: 0.8% Gaussia ?
-
 ?
1.13.12.5 cf3-coelenterazine + O2 relative activity to native coelenterazine: 16.8% Renilla reniformis ?
-
 ?
1.13.12.5 cf3-coelenterazine + O2 relative activity to native coelenterazine: 49.5% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 cf3-coelenterazine + O2 relative activity to native coelenterazine: 5% Renilla reniformis ?
-
 ?
1.13.12.5 coelenterazine + O2 relative activity: 100% Renilla reniformis ?
-
 ?
1.13.12.5 coelenterazine + O2 relative activity: 100% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 coelenterazine + O2 relative activity: 100% Gaussia ?
-
 ?
1.13.12.5 et-coelenterazine + O2 relative activity to native coelenterazine: 0.9% Gaussia ?
-
 ?
1.13.12.5 et-coelenterazine + O2 relative activity to native coelenterazine: 1.2% Renilla reniformis ?
-
 ?
1.13.12.5 et-coelenterazine + O2 relative activity to native coelenterazine: 21.1% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 et-coelenterazine + O2 relative activity to native coelenterazine: 78.3% Renilla reniformis ?
-
 ?
1.13.12.5 h-coelenterazine + O2 relative activity to native coelenterazine: 12.4% Renilla reniformis ?
-
 ?
1.13.12.5 h-coelenterazine + O2 relative activity to native coelenterazine: 5.8% Gaussia ?
-
 ?
1.13.12.5 h-coelenterazine + O2 relative activity to native coelenterazine: 54.1% Renilla reniformis ?
-
 ?
1.13.12.5 h-coelenterazine + O2 relative activity to native coelenterazine: 68.4% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 i-coelenterazine + O2 relative activity to native coelenterazine: 0.3% Renilla reniformis ?
-
 ?
1.13.12.5 i-coelenterazine + O2 relative activity to native coelenterazine: 0.9% Gaussia ?
-
 ?
1.13.12.5 i-coelenterazine + O2 relative activity to native coelenterazine: 32.3% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 i-coelenterazine + O2 relative activity to native coelenterazine: 5.1% Renilla reniformis ?
-
 ?
1.13.12.5 me-coelenterazine + O2 relative activity to native coelenterazine: 4.5% Gaussia ?
-
 ?
1.13.12.5 me-coelenterazine + O2 relative activity to native coelenterazine: 46.6% Oplophorus gracilirostris ?
-
 ?
1.13.12.5 me-coelenterazine + O2 relative activity to native coelenterazine: 5.2% Renilla reniformis ?
-
 ?
1.13.12.5 me-coelenterazine + O2 relative activity to native coelenterazine: 73.6% Renilla reniformis ?
-
 ?
1.13.12.5 meo-coelenterazine + O2 relative activity to native coelenterazine: 1.2% Renilla reniformis ?
-
 ?
1.13.12.5 meo-coelenterazine + O2 relative activity to native coelenterazine: 13.6% Gaussia ?
-
 ?
1.13.12.5 meo-coelenterazine + O2 relative activity to native coelenterazine: 18.2% Renilla reniformis ?
-
 ?
1.13.12.5 meo-coelenterazine + O2 relative activity to native coelenterazine: 68.1% Oplophorus gracilirostris ?
-
 ?
1.13.12.13 3iso-coelenterazine + O2 luminescence intesity (Imax): 78.2% Oplophorus gracilirostris oxidized 3iso-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 3me-coelenterazine + O2 luminescence intesity (Imax): 80% Oplophorus gracilirostris oxidized 3me-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 3meo-coelenterazine + O2 luminescence intesity (Imax): 189% Oplophorus gracilirostris oxidized 3meo-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 alphameh-coelenterazine + O2 luminescence intesity (Imax): 15.7% Oplophorus gracilirostris oxidized alphameh-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 cf3-coelenterazine + O2 luminescence intesity (Imax): 49.5% Oplophorus gracilirostris oxidized cf3-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 coelenterazine + O2 luminescence intesity (Imax): 100% Oplophorus gracilirostris oxidized coelenterazine + CO2 + hn
-
 ?
1.13.12.13 et-coelenterazine + O2 luminescence intesity (Imax): 21.5% Oplophorus gracilirostris oxidized et-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 h-coelenterazine + O2 luminescence intesity (Imax): 68.4% Oplophorus gracilirostris oxidized h-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 i-coelenterazine + O2 luminescence intesity (Imax): 32.3% Oplophorus gracilirostris oxidized i-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 me-coelenterazine + O2 luminescence intesity (Imax): 46.6% Oplophorus gracilirostris oxidized me-coelenterazine + CO2 + hn
-
 ?
1.13.12.13 meo-coelenterazine + O2 luminescence intesity (Imax): 68.1% Oplophorus gracilirostris oxidized meo-coelenterazine + CO2 + hn
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.5 19kOLase
-
 Oplophorus gracilirostris
1.13.12.5 GLase
-
 Gaussia
1.13.12.5 luciferase
-
 Renilla reniformis
1.13.12.5 luciferase
-
 Oplophorus gracilirostris
1.13.12.5 luciferase
-
 Gaussia
1.13.12.5 Oplophorus luciferase
-
 Oplophorus gracilirostris
1.13.12.5 Renilla luciferase
-
 Renilla reniformis
1.13.12.5 Renilla luciferase-547 red shift variant Renilla reniformis
1.13.12.5 RLase
-
 Renilla reniformis
1.13.12.5 RLase-547 red shift variant Renilla reniformis
1.13.12.13 19kOLase
-
 Oplophorus gracilirostris
1.13.12.13 luciferase
-
 Oplophorus gracilirostris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.5 7.4
-
 assay at Gaussia
1.13.12.5 7.6
-
 assay at Renilla reniformis
1.13.12.5 7.6
-
 assay at Oplophorus gracilirostris
1.13.12.13 7.6
-
 assay at Oplophorus gracilirostris