EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.31 | expressed in Escherichia coli as a His-tagged fusion protein | Oryctolagus cuniculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.11.31 | L183E/L192E | introduction of negatively charged residues at the intermonomer interface disturbs the hydrophobic dimer interaction of the wild-type LOX. Double mutant does not follow Michaelis-Menten kinetics. Double mutant are gradually inactivated at increasing substrate concentration | Oryctolagus cuniculus |
1.13.11.31 | W181E/H585E | introduction of negatively charged residues at the intermonomer interface disturbs the hydrophobic dimer interaction of the wild-type LOX. Double mutant does not follow Michaelis-Menten kinetics. Double mutant are gradually inactivated at increasing substrate concentration | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | 0.0213 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.31 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.31 | using Ni-NTA chromatography | Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.31 | linoleic acid + O2 | - |
Oryctolagus cuniculus | ? | - |
? | |
1.13.11.31 | additional information | binding of allosteric effector [13(S)-hydroxyoctadeca-9(Z),11(E)-dienoic acid] shifts the monomer-dimer equilibrium toward dimer formation | Oryctolagus cuniculus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.31 | dimer | binding of allosteric effector [13(S)-hydroxyoctadeca-9(Z),11(E)-dienoic acid] shifts the monomer-dimer equilibrium toward dimer formation. Enzyme dimerization may protect the enzyme from kinetic substrate inhibition by shielding the hydrophobic alpha2 helixes | Oryctolagus cuniculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.31 | LOX | - |
Oryctolagus cuniculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.31 | 47.23 | - |
linoleic acid | pH 7.4, 20°C, wild-type | Oryctolagus cuniculus |