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Literature summary extracted from

  • Duca, D.; Rose, D.R.; Glick, B.R.
    Characterization of a nitrilase and a nitrile hydratase from Pseudomonas sp. UW4 that converts indole-3-acetonitrile to produce indole-3-acetic acid (2014), Appl. Environ. Microbiol., 80, 4640-4649.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.84 additional information the enzyme does not require the help of a P47K activator protein to be active Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli DH5alpha cells Pseudomonas sp.
4.2.1.84 gene nthAB, DNA and amino acid sequence determination, analysis, and comparison, phylogenetic analysis and tree, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe2+ iron-type metalloenzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 32848
-
 x * 32848, calculated from amino acid sequence Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.84 indole-3-nitrile + H2O Pseudomonas sp.
-
 indole-3-acetamide the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Pseudomonas sp. K9NKH3
-
-
4.2.1.84 Pseudomonas sp. K9NKC3 gene nthA, alpha-subunit; gene nthAB
-
4.2.1.84 Pseudomonas sp. K9NLN7 gene nthB, beta-subunit; gene nthAB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 Ni Sepharose 6 column chromatography Pseudomonas sp.
4.2.1.84 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.84 culture condition optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C Pseudomonas sp.
-
4.2.1.84 additional information optimum growth temperature of the bacterium is 25°C to 30°C, but it is able to grow at 4°C Pseudomonas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 indole-3-acetonitrile + H2O
-
 Pseudomonas sp. indole-3-acetic acid + NH3
-
 ?
4.2.1.84 indole-3-nitrile + H2O
-
 Pseudomonas sp. indole-3-acetamide
-
 ?
4.2.1.84 indole-3-nitrile + H2O
-
 Pseudomonas sp. indole-3-acetamide the nitrile hydratase produces only indole-3-acetamide, no indole-3-acetic acid ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 32848, calculated from amino acid sequence Pseudomonas sp.
4.2.1.84 heterodimer alphabeta, nitrile hydratase alpha (NthA), and beta (NthB) subunits Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.5.1 NIT
-
 Pseudomonas sp.
4.2.1.84 NthAB
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 50
-
-
 Pseudomonas sp.
4.2.1.84 4
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.84 additional information
-
 maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C Pseudomonas sp.
4.2.1.84
-
 20 maximal activity at 4°C, over 50% of maximal activity at temperatures of 10 to 20°C, the activity decreases significantly as the temperature increases above 20°C Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 6
-
-
 Pseudomonas sp.
4.2.1.84 7.5
-
 narrow optimum, about 20% of maximal activity at pH 7.0 and about 40% at pH 8.0, profile overview Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.5.1 Pseudomonas sp. calculated from amino acid sequence
-
 5.7

General Information

EC Number General Information Comment Organism
4.2.1.84 metabolism the enzyme is important in the indole-3-acetic acid biosynthesis pathway together with the nitrilase, EC 3.5.5.1, which produces indole-3-amide, but also indole-3-acetic acid , overview Pseudomonas sp.