EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.2 | overexpressed in Escherichia coli | Carboxydothermus hydrogenoformans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.2 | 4.6 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
4.3.1.2 | 16 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.2 | K+ | required for maximum activity | Carboxydothermus hydrogenoformans | |
4.3.1.2 | Mg2+ | required for maximum activity | Carboxydothermus hydrogenoformans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 49000 | - |
SDS-PAGE | Carboxydothermus hydrogenoformans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.2 | Carboxydothermus hydrogenoformans | Q3AEU2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.2 | using metal affinity chromatography | Carboxydothermus hydrogenoformans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.2 | (2S,3S)-3-ethylaspartic acid | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | fumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + ethylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + hydrazine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + hydroxylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + methoxylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + methylamine | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | mesaconate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | methylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? | |
4.3.1.2 | propylfumarate + NH3 | - |
Carboxydothermus hydrogenoformans | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.2 | dimer | 2 * 49000 | Carboxydothermus hydrogenoformans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.1.2 | Ch-MAL | - |
Carboxydothermus hydrogenoformans |
4.3.1.2 | methylaspartate ammonia lyase | - |
Carboxydothermus hydrogenoformans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 70 | - |
- |
Carboxydothermus hydrogenoformans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 50 | - |
stable for more than 4 hours | Carboxydothermus hydrogenoformans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.2 | 16 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
4.3.1.2 | 78 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 9 | - |
- |
Carboxydothermus hydrogenoformans |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.1.2 | 4.9 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 30°C | Carboxydothermus hydrogenoformans | |
4.3.1.2 | 35 | - |
(2S,3S)-3-ethylaspartic acid | pH 9, 70°C | Carboxydothermus hydrogenoformans |