EC Number | Cloned (Comment) | Organism |
---|---|---|
5.5.1.13 | expressed in Escherichia coli C41 OverExpress cells | Arabidopsis thaliana |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.5.1.13 | enzyme complexed with (S)-15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate, vapor diffusion method, using 100 mM sodium citrate (pH 5.4), 30% (w/v) polyethylene glycol 400, 200 mM KH2PO4 | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.5.1.13 | D503A | the mutant exhibits a 7fold reduction in kcat | Arabidopsis thaliana |
5.5.1.13 | E211A | the mutation results in a nearly 500fold reduction in kcat | Arabidopsis thaliana |
5.5.1.13 | N425A | the mutant exhibits a 13fold reduction in kcat | Arabidopsis thaliana |
5.5.1.13 | R340A | the mutant exhibits an 850fold reduction in kcat | Arabidopsis thaliana |
5.5.1.13 | T421A | the mutant exhibits a 163fold reduction in kcat (KM is increased 2fold) | Arabidopsis thaliana |
5.5.1.13 | T421S | the mutant exhibits a 3fold reduction in kcat | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.13 | 0.0008 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425H, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.0017 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme E211A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.0018 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.002 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme R340A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.0029 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme D503A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.003 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421S, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.003 | - |
(E,E,E)-geranylgeranyl diphosphate | wild type enzyme, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.006 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421A, at pH 7.8 and 22°C | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.5.1.13 | Mg2+ | required for optimal catalytic activity | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.13 | (E,E,E)-geranylgeranyl diphosphate | Arabidopsis thaliana | - |
ent-copalyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.13 | Arabidopsis thaliana | Q38802 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.13 | Ni-NTA His-Bind resin column chromatography | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.13 | (E,E,E)-geranylgeranyl diphosphate | - |
Arabidopsis thaliana | ent-copalyl diphosphate | - |
? | |
5.5.1.13 | additional information | no activity with (S)-15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.13 | CPS | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.13 | 0.0011 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme R340A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.0019 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme E211A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.006 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.012 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425H, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.071 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.13 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme D503A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.3 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421S, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 0.9 | - |
(E,E,E)-geranylgeranyl diphosphate | wild type enzyme, at pH 7.8 and 22°C | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.13 | 0.55 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme R340A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 1 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 1.1 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme E211A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 20 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425H, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 40 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme N425A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 45 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme D503A, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 100 | - |
(E,E,E)-geranylgeranyl diphosphate | mutant enzyme T421S, at pH 7.8 and 22°C | Arabidopsis thaliana | |
5.5.1.13 | 300 | - |
(E,E,E)-geranylgeranyl diphosphate | wild type enzyme, at pH 7.8 and 22°C | Arabidopsis thaliana |