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Literature summary extracted from

  • Wang, Y.; Guo, E.W.; Yu, W.G.; Han, F.
    Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp. (2013), Biotechnol. Lett., 35, 703-708.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 AlCl3 53% inhibition at 1 mM Vibrio sp.
4.2.2.3 BaCl2 28% inhibition at 1 mM Vibrio sp.
4.2.2.3 CuCl2 97% inhibition at 1 mM Vibrio sp.
4.2.2.3 EDTA 96% inhibition at 1 mM Vibrio sp.
4.2.2.3 FeCl3 92% inhibition at 1 mM Vibrio sp.
4.2.2.3 NiCl2 33% inhibition at 1 mM Vibrio sp.
4.2.2.3 ZnCl2 58% inhibition at 1 mM Vibrio sp.
4.2.2.11 Al3+ 1 mM, 47% residual activity Vibrio sp.
4.2.2.11 AlCl3 53% inhibition at 1 mM Vibrio sp.
4.2.2.11 BaCl2 28% inhibition at 1 mM Vibrio sp.
4.2.2.11 Cu2+ 1 mM, 3% residual activity Vibrio sp.
4.2.2.11 CuCl2 97% inhibition at 1 mM Vibrio sp.
4.2.2.11 EDTA 1 mM, 6% residual activity; 96% inhibition at 1 mM Vibrio sp.
4.2.2.11 Fe3+ 1 mM, 8% residual activity Vibrio sp.
4.2.2.11 FeCl3 92% inhibition at 1 mM Vibrio sp.
4.2.2.11 NiCl2 33% inhibition at 1 mM Vibrio sp.
4.2.2.11 Zn2+ 1 mM, 42% residual activity Vibrio sp.
4.2.2.11 ZnCl2 58% inhibition at 1 mM Vibrio sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular
-
Vibrio sp.
-
-
4.2.2.11 extracellular
-
Vibrio sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 CaCl2 20% activation at 1 mM Vibrio sp.
4.2.2.3 MgCl2 15% activation at 1 mM Vibrio sp.
4.2.2.3 MnCl2 12% activation at 1 mM Vibrio sp.
4.2.2.3 additional information no effect on enzyme activity by SDS, K+, Li+, and NH4+ Vibrio sp.
4.2.2.11 Ca2+ 1 mM, 120% of initial activity Vibrio sp.
4.2.2.11 CaCl2 20% activation at 1 mM Vibrio sp.
4.2.2.11 Mg2+ 1 mM, 115% of initial activity Vibrio sp.
4.2.2.11 MgCl2 15% activation at 1 mM Vibrio sp.
4.2.2.11 Mn2+ 1 mM, 112% of initial activity Vibrio sp.
4.2.2.11 MnCl2 12% activation at 1 mM Vibrio sp.
4.2.2.11 additional information no effect on enzyme activity by SDS, K+, Li+, and NH4+ Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 37000
-
x * 37000, SDS-PAGE Vibrio sp.
4.2.2.11 37000
-
x * 37000, SDS-PAGE Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Vibrio sp.
-
-
-
4.2.2.3 Vibrio sp. QY105
-
-
-
4.2.2.11 Vibrio sp.
-
-
-
4.2.2.11 Vibrio sp.
-
QY105
-
4.2.2.11 Vibrio sp. QY105
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 native extracellular enzyme 23.6fold from cell culture supernatant Vibrio sp.
4.2.2.11 native extracellular enzyme 23.6fold from cell culture supernatant Vibrio sp.
4.2.2.11 purification from culture liquid Vibrio sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.11 culture fluid
-
Vibrio sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 2152
-
purified enzyme, pH 7.0, 38°C Vibrio sp.
4.2.2.11 2152
-
pH 7.0, 38°C Vibrio sp.
4.2.2.11 2152
-
purified enzyme, pH 7.0, 38°C Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. ?
-
?
4.2.2.3 additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. QY105 ?
-
?
4.2.2.3 poly(beta-D-mannuronate) about 30% activity compared to alginate Vibrio sp. ?
-
?
4.2.2.3 poly(beta-D-mannuronate) about 30% activity compared to alginate Vibrio sp. QY105 ?
-
?
4.2.2.3 sodium alginate high activity Vibrio sp. ?
-
?
4.2.2.3 sodium alginate high activity Vibrio sp. QY105 ?
-
?
4.2.2.11 alginate
-
Vibrio sp. ?
-
?
4.2.2.11 alginate
-
Vibrio sp. QY105 ?
-
?
4.2.2.11 additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. ?
-
?
4.2.2.11 additional information AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate Vibrio sp. QY105 ?
-
?
4.2.2.11 poly(alpha-(1->4)-L-guluronic acid) 108% of the activity with alginate Vibrio sp. ? main products are disaccharide, trisaccharide and tetrasaccharide ?
4.2.2.11 poly(alpha-L-guluronate) best substrate Vibrio sp. ?
-
?
4.2.2.11 poly-(beta-(1->4)-D-mannuronan) 30% of the activity with alginate Vibrio sp. ? main products are trisaccharide, tetrasaccharide and pentasaccharide ?
4.2.2.11 sodium alginate high activity Vibrio sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 37000, SDS-PAGE Vibrio sp.
4.2.2.11 ? x * 37000, SDS-PAGE Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
Vibrio sp.
4.2.2.3 AlyV5
-
Vibrio sp.
4.2.2.11 alginate lyase
-
Vibrio sp.
4.2.2.11 AlyV5
-
Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 38
-
-
Vibrio sp.
4.2.2.11 38
-
-
Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 90
-
purified native enzyme, 10 min, pH 7.0, 40% activity remians Vibrio sp.
4.2.2.11 90
-
10 min, 40% residual activity Vibrio sp.
4.2.2.11 90
-
purified native enzyme, 10 min, pH 7.0, 40% activity remains Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
-
Vibrio sp.
4.2.2.11 7
-
-
Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 6 9 purified native enzyme, 40°C, stable within this pH range Vibrio sp.
4.2.2.11 6 9
-
Vibrio sp.
4.2.2.11 6 9 purified native enzyme, 40°C, stable within this pH range Vibrio sp.