EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | AlCl3 | 53% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | BaCl2 | 28% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | CuCl2 | 97% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | EDTA | 96% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | FeCl3 | 92% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | NiCl2 | 33% inhibition at 1 mM | Vibrio sp. | |
4.2.2.3 | ZnCl2 | 58% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | Al3+ | 1 mM, 47% residual activity | Vibrio sp. | |
4.2.2.11 | AlCl3 | 53% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | BaCl2 | 28% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | Cu2+ | 1 mM, 3% residual activity | Vibrio sp. | |
4.2.2.11 | CuCl2 | 97% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | EDTA | 1 mM, 6% residual activity; 96% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | Fe3+ | 1 mM, 8% residual activity | Vibrio sp. | |
4.2.2.11 | FeCl3 | 92% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | NiCl2 | 33% inhibition at 1 mM | Vibrio sp. | |
4.2.2.11 | Zn2+ | 1 mM, 42% residual activity | Vibrio sp. | |
4.2.2.11 | ZnCl2 | 58% inhibition at 1 mM | Vibrio sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.2.3 | extracellular | - |
Vibrio sp. | - |
- |
4.2.2.11 | extracellular | - |
Vibrio sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | CaCl2 | 20% activation at 1 mM | Vibrio sp. | |
4.2.2.3 | MgCl2 | 15% activation at 1 mM | Vibrio sp. | |
4.2.2.3 | MnCl2 | 12% activation at 1 mM | Vibrio sp. | |
4.2.2.3 | additional information | no effect on enzyme activity by SDS, K+, Li+, and NH4+ | Vibrio sp. | |
4.2.2.11 | Ca2+ | 1 mM, 120% of initial activity | Vibrio sp. | |
4.2.2.11 | CaCl2 | 20% activation at 1 mM | Vibrio sp. | |
4.2.2.11 | Mg2+ | 1 mM, 115% of initial activity | Vibrio sp. | |
4.2.2.11 | MgCl2 | 15% activation at 1 mM | Vibrio sp. | |
4.2.2.11 | Mn2+ | 1 mM, 112% of initial activity | Vibrio sp. | |
4.2.2.11 | MnCl2 | 12% activation at 1 mM | Vibrio sp. | |
4.2.2.11 | additional information | no effect on enzyme activity by SDS, K+, Li+, and NH4+ | Vibrio sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 37000 | - |
x * 37000, SDS-PAGE | Vibrio sp. |
4.2.2.11 | 37000 | - |
x * 37000, SDS-PAGE | Vibrio sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.3 | Vibrio sp. | - |
- |
- |
4.2.2.3 | Vibrio sp. QY105 | - |
- |
- |
4.2.2.11 | Vibrio sp. | - |
- |
- |
4.2.2.11 | Vibrio sp. | - |
QY105 | - |
4.2.2.11 | Vibrio sp. QY105 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.3 | native extracellular enzyme 23.6fold from cell culture supernatant | Vibrio sp. |
4.2.2.11 | native extracellular enzyme 23.6fold from cell culture supernatant | Vibrio sp. |
4.2.2.11 | purification from culture liquid | Vibrio sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.2.11 | culture fluid | - |
Vibrio sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 2152 | - |
purified enzyme, pH 7.0, 38°C | Vibrio sp. |
4.2.2.11 | 2152 | - |
pH 7.0, 38°C | Vibrio sp. |
4.2.2.11 | 2152 | - |
purified enzyme, pH 7.0, 38°C | Vibrio sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.3 | additional information | AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate | Vibrio sp. | ? | - |
? | |
4.2.2.3 | additional information | AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate | Vibrio sp. QY105 | ? | - |
? | |
4.2.2.3 | poly(beta-D-mannuronate) | about 30% activity compared to alginate | Vibrio sp. | ? | - |
? | |
4.2.2.3 | poly(beta-D-mannuronate) | about 30% activity compared to alginate | Vibrio sp. QY105 | ? | - |
? | |
4.2.2.3 | sodium alginate | high activity | Vibrio sp. | ? | - |
? | |
4.2.2.3 | sodium alginate | high activity | Vibrio sp. QY105 | ? | - |
? | |
4.2.2.11 | alginate | - |
Vibrio sp. | ? | - |
? | |
4.2.2.11 | alginate | - |
Vibrio sp. QY105 | ? | - |
? | |
4.2.2.11 | additional information | AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate | Vibrio sp. | ? | - |
? | |
4.2.2.11 | additional information | AlyV5 shows activities towards both polyguluronate and polymannuronate, but degrades the former more efficiently. AlyV5 mainly produces disaccharide, trisaccharide and tetrasaccharide from polyguluronate, trisaccharide, tetrasaccharide and pentasaccharide from polymannuronate | Vibrio sp. QY105 | ? | - |
? | |
4.2.2.11 | poly(alpha-(1->4)-L-guluronic acid) | 108% of the activity with alginate | Vibrio sp. | ? | main products are disaccharide, trisaccharide and tetrasaccharide | ? | |
4.2.2.11 | poly(alpha-L-guluronate) | best substrate | Vibrio sp. | ? | - |
? | |
4.2.2.11 | poly-(beta-(1->4)-D-mannuronan) | 30% of the activity with alginate | Vibrio sp. | ? | main products are trisaccharide, tetrasaccharide and pentasaccharide | ? | |
4.2.2.11 | sodium alginate | high activity | Vibrio sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.3 | ? | x * 37000, SDS-PAGE | Vibrio sp. |
4.2.2.11 | ? | x * 37000, SDS-PAGE | Vibrio sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.3 | alginate lyase | - |
Vibrio sp. |
4.2.2.3 | AlyV5 | - |
Vibrio sp. |
4.2.2.11 | alginate lyase | - |
Vibrio sp. |
4.2.2.11 | AlyV5 | - |
Vibrio sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 38 | - |
- |
Vibrio sp. |
4.2.2.11 | 38 | - |
- |
Vibrio sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 90 | - |
purified native enzyme, 10 min, pH 7.0, 40% activity remians | Vibrio sp. |
4.2.2.11 | 90 | - |
10 min, 40% residual activity | Vibrio sp. |
4.2.2.11 | 90 | - |
purified native enzyme, 10 min, pH 7.0, 40% activity remains | Vibrio sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 7 | - |
- |
Vibrio sp. |
4.2.2.11 | 7 | - |
- |
Vibrio sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 6 | 9 | purified native enzyme, 40°C, stable within this pH range | Vibrio sp. |
4.2.2.11 | 6 | 9 | - |
Vibrio sp. |
4.2.2.11 | 6 | 9 | purified native enzyme, 40°C, stable within this pH range | Vibrio sp. |