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Literature summary extracted from

  • Golyshina, O.V.; Golyshin, P.N.; Timmis, K.N.; Ferrer, M.
    The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum (2006), Environ. Microbiol., 8, 416-425.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expression in Escherichia coli Ferroplasma acidiphilum
3.2.1.20 expression in Escherichia coli Ferroplasma acidiphilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.45
-
 4-nitrophenyl propionate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 0.47
-
 4-nitrophenyl acetate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 0.6
-
 4-nitrophenyl butyrate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 2.2
-
 Triacetin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 3.5
-
 tripropionin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 3.9
-
 4-nitrophenyl hexanoate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 7.4
-
 tributyrin pH 2.0, 50°C Ferroplasma acidiphilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 membrane
-
 Ferroplasma acidiphilum 16020
-
3.2.1.20 intracellular
-
 Ferroplasma acidiphilum 5622
-
3.2.1.20 membrane
-
 Ferroplasma acidiphilum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 34734
-
 1 * 34734, calculated from sequence Ferroplasma acidiphilum
3.1.1.1 35000
-
 non-denasturing PAGE Ferroplasma acidiphilum
3.1.1.1 36000
-
 1 * 36000, SDS-PAGE Ferroplasma acidiphilum
3.2.1.20 28316
-
 1 * 28316, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 40505
-
 1 * 40505, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 42000
-
 1 * 42000, SDS-PAGE Ferroplasma acidiphilum
3.2.1.20 56000
-
 non-denasturing PAGE Ferroplasma acidiphilum
3.2.1.20 57000
-
 non-denaturing PAGE Ferroplasma acidiphilum
3.2.1.20 57300
-
 1 * 57300, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 80000
-
 non-denasturing PAGE Ferroplasma acidiphilum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Ferroplasma acidiphilum Q2PCE5
-
-
3.2.1.20 Ferroplasma acidiphilum Q2PCE2
-
-
3.2.1.20 Ferroplasma acidiphilum Q2PCE3
-
-
3.2.1.20 Ferroplasma acidiphilum Q5K3Q3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 purified from both Escherichia coli and Ferroplasma acidiphilum Ferroplasma acidiphilum
3.2.1.20 purified from both Escherichia coli and Ferroplasma acidiphilum Ferroplasma acidiphilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Ferroplasma acidiphilum 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Ferroplasma acidiphilum 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 Ferroplasma acidiphilum 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O
-
 Ferroplasma acidiphilum 4-nitrophenol + propionate
-
 ?
3.1.1.1 additional information no activity with triolein Ferroplasma acidiphilum ?
-
 ?
3.1.1.1 triacetin + H2O
-
 Ferroplasma acidiphilum ?
-
 ?
3.1.1.1 tributyrin + H2O
-
 Ferroplasma acidiphilum ?
-
 ?
3.1.1.1 tripropionin + H2O
-
 Ferroplasma acidiphilum ?
-
 ?
3.2.1.20 maltose + H2O
-
 Ferroplasma acidiphilum 2 D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O very low activity Ferroplasma acidiphilum ?
-
 ?
3.2.1.20 maltotriose + H2O
-
 Ferroplasma acidiphilum ?
-
 ?
3.2.1.20 sucrose + H2O
-
 Ferroplasma acidiphilum D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 36000, SDS-PAGE Ferroplasma acidiphilum
3.1.1.1 monomer 1 * 34734, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 ? 1 * 42000, SDS-PAGE Ferroplasma acidiphilum
3.2.1.20 ? 1 * 28316, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 ? 1 * 40505, calculated from sequence Ferroplasma acidiphilum
3.2.1.20 ? 1 *28000, SDS-PAGE Ferroplasma acidiphilum
3.2.1.20 monomer 1 * 57000, SDS-PAGE Ferroplasma acidiphilum
3.2.1.20 monomer 1 * 57300, calculated from sequence Ferroplasma acidiphilum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EStFa
-
 Ferroplasma acidiphilum
3.2.1.20 alphaGluFa
-
 Ferroplasma acidiphilum
3.2.1.20 GlyFa1
-
 Ferroplasma acidiphilum
3.2.1.20 GlyFa2
-
 Ferroplasma acidiphilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 50
-
 pH 1.0-2.5, hydrolysis of 4-nitrophenol propionate Ferroplasma acidiphilum
3.2.1.20 60
-
-
 Ferroplasma acidiphilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 50
-
 pH 1.7-2.0, half-life: 48 min Ferroplasma acidiphilum
3.2.1.20 60
-
 pH 1.7-2.5, half-life: 23 min Ferroplasma acidiphilum
3.2.1.20 60
-
 pH 2.5-3.5, half-life: 34 min Ferroplasma acidiphilum
3.2.1.20 60
-
 pH 3.5-4.0, half-life: 12 min Ferroplasma acidiphilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 2
-
 4-nitrophenyl hexanoate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 10
-
 4-nitrophenyl butyrate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 23
-
 tributyrin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 25
-
 4-nitrophenyl acetate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 29
-
 4-nitrophenyl propionate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 44
-
 tripropionin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 47
-
 Triacetin pH 2.0, 50°C Ferroplasma acidiphilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 1.5
-
-
 Ferroplasma acidiphilum
3.2.1.20 2
-
-
 Ferroplasma acidiphilum
3.2.1.20 2.5 3
-
 Ferroplasma acidiphilum
3.2.1.20 3.5 4
-
 Ferroplasma acidiphilum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 1 3 pH 1.0: about 70% of maximal activity, pH 3.0: about 65% of maximal activity Ferroplasma acidiphilum
3.2.1.20 1 4 pH 1.0: about 45% of maximal activity, pH 4.0: about 55% of maximal activity Ferroplasma acidiphilum
3.2.1.20 1.5 5 pH 1.5: about 70% of maximal activity, pH 5.0: about 60% of maximal activity Ferroplasma acidiphilum
3.2.1.20 2.5 5.5 pH 2.5: about 40% of maximal activity, pH 5.5: about 40% of maximal activity Ferroplasma acidiphilum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Ferroplasma acidiphilum calculated from sequence
-
 5.91
3.2.1.20 Ferroplasma acidiphilum calculated from sequence
-
 6.42
3.2.1.20 Ferroplasma acidiphilum calculated from sequence
-
 9.53

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 0.6
-
 4-nitrophenyl hexanoate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 3.1
-
 tributyrin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 12.7
-
 tripropionin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 16.7
-
 4-nitrophenyl butyrate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 21.3
-
 Triacetin pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 53
-
 4-nitrophenyl acetate pH 2.0, 50°C Ferroplasma acidiphilum
3.1.1.1 64.2
-
 4-nitrophenyl propionate pH 2.0, 50°C Ferroplasma acidiphilum
3.2.1.20 0.1
-
 maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 0.3
-
 maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 0.6
-
 maltotetraose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 18.8
-
 maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 28
-
 maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 37
-
 maltotriose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 73
-
 maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 101
-
 maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 142
-
 sucrose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 197
-
 maltose 60°C, pH not specified in the publication Ferroplasma acidiphilum
3.2.1.20 293
-
 sucrose 60°C, pH not specified in the publication Ferroplasma acidiphilum