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Literature summary extracted from

  • Giraldo, M.A.; Goncalves, H.B.; Furriel, R.d.o.s..P.; Jorge, J.A.; Guimaraes, L.H.
    Characterization of the co-purified invertase and beta-glucosidase of a multifunctional extract from Aspergillus terreus (2014), World J. Microbiol. Biotechnol., 30, 1501-1510.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 2-mercaptoethanol 134.4% activity at 1 mM Aspergillus terreus
3.2.1.26 NaBr 130.9% activity at 1 mM Aspergillus terreus
3.2.1.26 NaCl 120% activity at 1 mM Aspergillus terreus
3.2.1.26 NH4F 130.5% activity at 1 mM Aspergillus terreus
3.2.1.26 Zn(NO3)2 155.4% activity at 1 mM Aspergillus terreus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 2-mercaptoethanol 87.8% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Ag+ 18% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Al3+ 20.5% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Co2+ 63.5% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Cu2+ 28.5% residual activity at 10 mM Aspergillus terreus
3.2.1.26 EDTA 47.9% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Fe2+ 11.6% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Fe3+ 16.5% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Hg+ complete inhibition at 10 mM Aspergillus terreus
3.2.1.26 K+ 67.1% residual activity at 10 mM Aspergillus terreus
3.2.1.26 NaBr 62.7% residual activity at 10 mM Aspergillus terreus
3.2.1.26 NaH2PO4 67.9% residual activity at 10 mM Aspergillus terreus
3.2.1.26 NH4Cl 36.6% residual activity at 10 mM Aspergillus terreus
3.2.1.26 NH4F 51.3% residual activity at 10 mM Aspergillus terreus
3.2.1.26 Zn(NO3)2 66.2% residual activity at 10 mM Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 11.9
-
 sucrose in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus
3.2.1.26 22.5
-
 cellobiose in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus
3.2.1.26 22.7
-
 sucrose in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus
3.2.1.26 123.6
-
 cellobiose in the presence of Mn2+, in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.26 Ba2+ 136% activity at 1 mM Aspergillus terreus
3.2.1.26 Ca2+ 145% activity at 10 mM Aspergillus terreus
3.2.1.26 Co2+ 168% activity at 1 mM Aspergillus terreus
3.2.1.26 Fe3+ 117% activity at 1 mM Aspergillus terreus
3.2.1.26 K+ 124% activity at 1 mM Aspergillus terreus
3.2.1.26 Mg2+ 161% activity at 1 mM Aspergillus terreus
3.2.1.26 Mn2+ 261% activity at 1 mM Aspergillus terreus
3.2.1.26 Zn2+ 126% activity at 1 mM Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 26900
-
 1 * 46700 + 1 * 26900, SDS-PAGE Aspergillus terreus
3.2.1.26 46700
-
 1 * 46700 + 1 * 26900, SDS-PAGE Aspergillus terreus
3.2.1.26 74800
-
 gel filtration Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Aspergillus terreus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 culture condition:rye flour-grown cell
-
 Aspergillus terreus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 3.9
-
 crude extract, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus
3.2.1.26 555
-
 after 139.4fold purification, with sucrose as substrate, in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 cellobiose + H2O
-
 Aspergillus terreus ?
-
 ?
3.2.1.26 inulin + H2O worst substrate Aspergillus terreus ?
-
 ?
3.2.1.26 additional information no activity with maltose, starch, and trehalose Aspergillus terreus ?
-
 ?
3.2.1.26 raffinose + H2O
-
 Aspergillus terreus D-fructose + melibiose
-
 ?
3.2.1.26 sucrose + H2O best substrate Aspergillus terreus D-fructose + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 heterodimer 1 * 46700 + 1 * 26900, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 invertase
-
 Aspergillus terreus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.26 55 65
-
 Aspergillus terreus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 55 70 the enzyme remains stable for 1 h at 55°C when diluted in water. The extracellular invertase is fully stable at lower temperatures, but half-lives of 40 and 5 min are estimated at 60 and 70°C, respectively Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.26 4 6
-
 Aspergillus terreus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.26 3 10 the enzyme remains stable for 1 h Aspergillus terreus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.26 1
-
 in 100 mM sodium acetate buffer, pH 5.0, at 50°C Aspergillus terreus Hg+