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Literature summary extracted from

  • Zeiser, J.; Muehlenbeck, L.H.; Schweiger, P.; Deppenmeier, U.
    Characterization of a periplasmic quinoprotein from Sphingomonas wittichii that functions as aldehyde dehydrogenase (2014), Appl. Microbiol. Biotechnol., 98, 2067-2079.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.5.2 expression in Escherichia coli Rhizorhabdus wittichii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.5.2 CuSO4 10 mM, almost complete inhibition Rhizorhabdus wittichii
1.2.5.2 EDTA 1 mM, 25% decrease in activity Rhizorhabdus wittichii
1.2.5.2 FeCl3 10 mM, almost complete inhibition Rhizorhabdus wittichii
1.2.5.2 ZnSO4 10 mM, almost complete inhibition Rhizorhabdus wittichii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.5.2 12.3
-
 Butanal pH 8, 25°C Rhizorhabdus wittichii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.5.2 periplasm
-
 Rhizorhabdus wittichii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.5.2 39300
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
1.2.5.2 39300
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
1.2.5.2 39329
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
1.2.5.2 39329
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
1.2.5.2 39400
-
 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
1.2.5.2 39400
-
 minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii
1.2.5.2 330000
-
 PAGE, minor protein band Rhizorhabdus wittichii
1.2.5.2 450000
-
 PAGE Rhizorhabdus wittichii

Organism

EC Number Organism UniProt Comment Textmining
1.2.5.2 Rhizorhabdus wittichii A5VEL7
-
-
1.2.5.2 Rhizorhabdus wittichii DSM 6014 A5VEL7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.5.2 180
-
 substrate L-sorbosone, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1050
-
 substrate glyoxal, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1060
-
 substrate glycolaldehyde, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1170
-
 substrate 3-methylbenzaldehyde, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1450
-
 substrate valeraldehyde, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1690
-
 substrate methylglyoxal, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1740
-
 substrate propanal, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 1900
-
 substrate phenylglyoxal, pH 8, 25°C Rhizorhabdus wittichii
1.2.5.2 2470
-
 substrate butanal, pH 8, 25°C Rhizorhabdus wittichii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.5.2 3-methylbenzaldehyde + ferricyanide
-
 Rhizorhabdus wittichii 3-methylbenzoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 3-methylbenzaldehyde + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 3-methylbenzoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 butanal + ferricyanide
-
 Rhizorhabdus wittichii butanoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 butanal + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 butanoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 glycolaldehyde + ferricyanide
-
 Rhizorhabdus wittichii hydroxyacetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 glycolaldehyde + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 hydroxyacetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 glyoxal + ferricyanide
-
 Rhizorhabdus wittichii oxoacetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 glyoxal + ferricyanide
-
 Rhizorhabdus wittichii DSM 6014 oxoacetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 L-sorbosone + ferricyanide
-
 Rhizorhabdus wittichii ? + ferrocyanide + H+
-
 ?
1.2.5.2 methylglyoxal + ferricyanide
-
 Rhizorhabdus wittichii 2-oxopropanoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 additional information the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids Rhizorhabdus wittichii ?
-
 ?
1.2.5.2 additional information the enzyme can also transfer electrons to the electron acceptor system DCPIP/PMS, but the activity is only 50% of the rate obtained with ferricyanide. Enzyme displays a broad substrate spectrum and oxidizes short- and medium-chain aliphatic aldehydes (C1-C6), ketoaldehydes, dialdehydes; some aromatic aldehydes and hydroxyaldehydes to the corresponding carboxylic acids Rhizorhabdus wittichii DSM 6014 ?
-
 ?
1.2.5.2 phenylglyoxal + ferricyanide
-
 Rhizorhabdus wittichii oxo(phenyl) acetic acid + ferrocyanide + H+
-
 ?
1.2.5.2 propanal + ferricyanide
-
 Rhizorhabdus wittichii propanoic acid + ferrocyanide + H+
-
 ?
1.2.5.2 valeraldehyde + ferricyanide
-
 Rhizorhabdus wittichii pentanoic acid + ferrocyanide + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.5.2 dodecamer 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus some octamer Rhizorhabdus wittichii
1.2.5.2 octamer minor protein band, 12 * 39400, SDS-PAGE, 12 * 39300, calculated, 12 * 39329, MALDI-TOF, plus dodecamer Rhizorhabdus wittichii

Synonyms

EC Number Synonyms Comment Organism
1.2.5.2 glucose sorbosone dehydrogenase
-
 Rhizorhabdus wittichii
1.2.5.2 Swit_4395
-
 Rhizorhabdus wittichii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.5.2 30
-
-
 Rhizorhabdus wittichii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.5.2 9.5
-
-
 Rhizorhabdus wittichii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.5.2 9.5
-
 half-life below 5 min Rhizorhabdus wittichii

General Information

EC Number General Information Comment Organism
1.2.5.2 physiological function overproduction of enzyme Swit_4395 in Sphongomonas wittichii RW1 dramatically increases the tolerance of the bacterium toward butyraldehyde and thus might contribute to the detoxification of toxic aldehydes Rhizorhabdus wittichii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.5.2 43
-
 Butanal pH 8, 25°C Rhizorhabdus wittichii