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Literature summary extracted from

  • Eklund, S.; Lindas, A.C.; Hamnevik, E.; Widersten, M.; Tomkinson, B.
    Exploring the active site of tripeptidyl-peptidase II through studies of pH dependence of reaction kinetics (2012), Biochim. Biophys. Acta, 1824, 561-570.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.14.10 expressed in Escherichia coli Drosophila melanogaster
3.4.14.10 expressed in Escherichia coli Mus musculus
3.4.14.10 expressed in Escherichia coli Homo sapiens
3.4.14.10 expression in Escherichia coli Drosophila melanogaster
3.4.14.10 expression of murine TPP2 gene optimized for high-level expression in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.14.10 D387G mutant shows an increase in pKa for kcat Drosophila melanogaster
3.4.14.10 D387G mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group Mus musculus
3.4.14.10 D387G the pKa values of mutant D387G for kcat (app) increase when Asp-387 is changed to glycine. Mutant shows a bell-shaped pH-dependence of kcat(app), possibly due to an impaired protonation of the leaving group. Mutant shows a decresed kcat for all substrates and a highly decreased kcat/Km compared to wild-type. In contrast to wild-type at higher pH the kcat (app) value decreases Drosophila melanogaster
3.4.14.10 E331Q mutant has a 2-3 orders of magnitude decreased kcat app/KM compared to wild-type, mostly dependent on an increase in KM Mus musculus
3.4.14.10 G375D less than 0.1% of wild-type activity Mus musculus
3.4.14.10 G375D mutant has only 0.1% activity of wild-type at pH 7.5. Mutant shows a marked decrease in pH optimum compared to wild-type Mus musculus
3.4.14.10 H267A mutant shows bell-shaped pH-dependence of kcat, possibly due to an impaired protonation of the leaving group Mus musculus
3.4.14.10 H267A mutant shows mostly a decresed kcat for all substrates and a highly decreased kcat/Km between 2 to fold orders of magnitude compared to wild-type. kcat shows a higher rise with pH in mutant H267A than in wild-type with substrate Ala-Ala-Phe-4-nitroanilide but at pH more than 7.6 kcat (app) decreases for substrate Ala-Ala-Phe-4-nitroanilide, resulting in a significantly better fit for a bell-shaped curve than for a sigmoidal Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.10 0.0036
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.008
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 0.008
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 0.009
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 0.009
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 0.01
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 0.01
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 0.0106
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.012
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.012
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.012
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.012
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.013
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.013
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.013
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.013
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.014
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 0.014
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 0.015
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.015
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.017
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 0.017
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.017
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.017
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 0.024
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.024
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.024
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 0.024
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 0.028
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 0.028
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 0.03
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.03
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.03
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.03
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.036
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.037
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 0.037
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 0.039
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.039
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.042
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.042
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.05
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.05
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 0.05
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.05
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.054
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 0.054
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3.4.14.10 0.054
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 0.054
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 0.056
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3.4.14.10 0.056
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 0.057
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 0.057
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 0.058
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.058
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.06
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.06
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.06
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.06
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.06
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.06
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.062
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.062
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.062
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 0.062
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 0.07
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 0.07
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.074
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.074
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.09
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.09
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.09
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 0.09
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 0.1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 0.1
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.11
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.11
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.13
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.2
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.2
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.21
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.21
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.25
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.25
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.27
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.27
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.3
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.33
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 0.6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 0.6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 1.19
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 3.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
3.4.14.10 5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 20
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 30
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.10 Drosophila melanogaster
-
-
-
3.4.14.10 Drosophila melanogaster Q9V6K1
-
-
3.4.14.10 Homo sapiens
-
-
-
3.4.14.10 Mus musculus
-
-
-
3.4.14.10 Mus musculus Q64514
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.10 using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Drosophila melanogaster
3.4.14.10 using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Mus musculus
3.4.14.10 using polyethyleneimine precipitation, (NH4)2SO4 precipitation, dialysis, anion-exchange chromatography, hydrophobic interaction chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.10 commercial preparation
-
 Homo sapiens
-
3.4.14.10 erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.10 Ala-Ala-Ala-4-nitroanilide + H2O
-
 Drosophila melanogaster Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.10 Ala-Ala-Ala-4-nitroanilide + H2O
-
 Mus musculus Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.10 Ala-Ala-Ala-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.10 Ala-Ala-Phe-4-nitroanilide + H2O
-
 Drosophila melanogaster Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.14.10 Ala-Ala-Phe-4-nitroanilide + H2O
-
 Mus musculus Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.14.10 Ala-Ala-Phe-4-nitroanilide + H2O
-
 Homo sapiens Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Homo sapiens L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Mus musculus L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Ala 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Drosophila melanogaster L-Ala-L-Ala-L-Ala + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Homo sapiens L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Mus musculus L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?
3.4.14.10 L-Ala-L-Ala-L-Phe 4-nitroanilide + H2O both the KM and kcat are lower for cleavage of L-Ala-L-Ala-L-Ala 4-nitroanilide than for L-Ala-L-Ala-L-Phe 4-nitroanilide, the former possibly can bind non-productively to the active site of the enzyme Drosophila melanogaster L-Ala-L-Ala-L-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.10 ? x * 100000-140000, SDS-PAGE of recombinant protein, full-length TPP II and shortened versions thereof Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.14.10 dTPP II
-
 Drosophila melanogaster
3.4.14.10 hTPP II
-
 Homo sapiens
3.4.14.10 mTPP II
-
 Mus musculus
3.4.14.10 tripeptidyl aminopeptidase II
-
 Drosophila melanogaster
3.4.14.10 tripeptidyl aminopeptidase II
-
 Mus musculus
3.4.14.10 tripeptidyl aminopeptidase II
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.10 37
-
 assay at Drosophila melanogaster
3.4.14.10 37
-
 assay at Mus musculus
3.4.14.10 37
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.10 0.25
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 0.25
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.38
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 0.38
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.441
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 0.441
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.509
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 0.509
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.58
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 0.58
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 0.74
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.74
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.857
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 0.857
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.86
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 0.86
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 0.9
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 0.9
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.9
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 1
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 1.12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 1.12
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 1.29
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 1.29
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 1.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 1.3
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 1.42
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 1.42
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 1.5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 1.5
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 2
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 2
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 3
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 3
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 3.1
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 3.1
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 3.3
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 3.3
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 4
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 4
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 4
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 4
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 4.2
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 5
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 5
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 6
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 7.2
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 7.2
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 9.6
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 9.6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 10
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 10
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
3.4.14.10 10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 11
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 12
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 12
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 12
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 13
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 13
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 13
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 18
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 19
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 19
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 21
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 21
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 22
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 22
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 27
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 27
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 27
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 27
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 30
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 30
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3.4.14.10 33
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 33
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 33
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 33
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 38
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 38
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 42
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 42
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 50
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 50
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 56.8
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 57
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 57
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 58.8
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 60
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 60
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 65
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 65
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 80
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 96
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 96
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3.4.14.10 100
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 114
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 114
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 125
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.10 7.5
-
 assay at Drosophila melanogaster
3.4.14.10 7.5
-
 assay at Mus musculus
3.4.14.10 7.5
-
 assay at Homo sapiens
3.4.14.10 7.6
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Drosophila melanogaster
3.4.14.10 7.9
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Mus musculus
3.4.14.10 7.9
-
 optimal pH, substrate: Ala-Ala-Phe-4-nitroanilide Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.10 additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Homo sapiens
3.4.14.10 additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Mus musculus
3.4.14.10 additional information
-
 isoform TPP II has a bell-shaped pH dependence of kcat app/KM probably due to deprotonation of the N-terminal amino group of the substrate at higher pH Drosophila melanogaster
3.4.14.10 additional information
-
 kcat(app) increases with pH Drosophila melanogaster
3.4.14.10 additional information
-
 kcat(app) increases with pH Mus musculus
3.4.14.10 additional information
-
 kcat(app) increases with pH Homo sapiens
3.4.14.10 7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Drosophila melanogaster
3.4.14.10 7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Mus musculus
3.4.14.10 7 8 optimal pH, substrate: Ala-Ala-Ala-4-nitroanilide Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.10 0.0000038
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000005
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000006
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000006
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000007
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.00001
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.00001
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000011
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000012
-
 Ala-Ala-Phe-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000013
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000014
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C, mutant H267A Mus musculus
3.4.14.10 0.0000165
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant H267A Mus musculus
3.4.14.10 0.00002
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant H267A Mus musculus
3.4.14.10 0.000021
-
 Ala-Ala-Ala-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000021
-
 Ala-Ala-Ala-4-nitroanilide pH 7.3, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000022
-
 Ala-Ala-Phe-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000023
-
 Ala-Ala-Phe-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.00003
-
 Ala-Ala-Phe-4-nitroanilide pH 6.2, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.00003
-
 Ala-Ala-Ala-4-nitroanilide pH 6.7, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000036
-
 Ala-Ala-Ala-4-nitroanilide pH 7.0, 37°C, mutant D387G Drosophila melanogaster
3.4.14.10 0.000138
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.00016
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.00019
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.00023
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 0.00023
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 0.00025
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 0.00028
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.0003
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.0003
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.00031
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 0.00033
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 0.00033
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 0.00037
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 0.0005
-
 Ala-Ala-Ala-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.00056
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 0.00056
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.00082
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.0009
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 0.00098
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.001
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 0.0015
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 0.0018
-
 Ala-Ala-Phe-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 0.0025
-
 Ala-Ala-Phe-4-nitroanilide pH 8.3, 37°C Mus musculus
3.4.14.10 0.0026
-
 Ala-Ala-Ala-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 0.0028
-
 Ala-Ala-Phe-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 0.0029
-
 Ala-Ala-Ala-4-nitroanilide pH 6.4, 37°C Mus musculus
3.4.14.10 0.0031
-
 Ala-Ala-Phe-4-nitroanilide pH 7.9, 37°C Mus musculus
3.4.14.10 0.0035
-
 Ala-Ala-Phe-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 0.0038
-
 Ala-Ala-Ala-4-nitroanilide pH 7.6, 37°C Mus musculus
3.4.14.10 0.0039
-
 Ala-Ala-Ala-4-nitroanilide pH 6.8, 37°C Mus musculus
3.4.14.10 3.4
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 3.82
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 5
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.4, 37°C Mus musculus
3.4.14.10 6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 6
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 8.3, 37°C Mus musculus
3.4.14.10 6.7
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 7.1
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 8
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 9
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
3.4.14.10 9.6
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 10
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 10
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 11
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 12
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 13
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 6.8, 37°C Mus musculus
3.4.14.10 14
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 8.3, 37°C Mus musculus
3.4.14.10 15
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.4, 37°C Mus musculus
3.4.14.10 15
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.9, 37°C Mus musculus
3.4.14.10 16.5
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant H267A, pH 7.6, 37°C Mus musculus
3.4.14.10 17
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 6.8, 37°C Mus musculus
3.4.14.10 20
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant E331Q, pH 7.6, 37°C Mus musculus
3.4.14.10 20
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant H267A, pH 7.9, 37°C Mus musculus
3.4.14.10 21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 21
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 22
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 23
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 30
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide mutant D387G, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 30
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 36
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide mutant D387G, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 138
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 160
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 190
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 230
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.4, 37°C Homo sapiens
3.4.14.10 250
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 8.3, 37°C Homo sapiens
3.4.14.10 280
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 300
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 300
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 310
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 6.8, 37°C Homo sapiens
3.4.14.10 330
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.9, 37°C Homo sapiens
3.4.14.10 330
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Drosophila melanogaster
3.4.14.10 370
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide pH 7.6, 37°C Homo sapiens
3.4.14.10 500
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 560
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 560
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Drosophila melanogaster
3.4.14.10 820
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Drosophila melanogaster
3.4.14.10 980
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 1000
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Drosophila melanogaster
3.4.14.10 1500
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Drosophila melanogaster
3.4.14.10 1800
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 2100
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.4, 37°C Mus musculus
3.4.14.10 2500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 2600
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 8.3, 37°C Mus musculus
3.4.14.10 2800
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 2900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 6.8, 37°C Mus musculus
3.4.14.10 3100
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 3500
-
 L-Ala-L-Ala-L-Phe 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus
3.4.14.10 3800
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.9, 37°C Mus musculus
3.4.14.10 3900
-
 L-Ala-L-Ala-L-Ala 4-nitroanilide wild-type, pH 7.6, 37°C Mus musculus