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Literature summary extracted from

  • Jagtap, S.; Dhiman, S.; Kim, T.; Li, J.; Chan Kang, Y.; Lee, J.
    Characterization of a beta-1,4-glucosidase from a newly isolated strain of Pholiota adiposa and its application to the hydrolysis of biomass (2013), Biomass Bioenergy, 54, 181-190.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 ethanol at up to 15% v/v Pholiota adiposa
3.2.1.21 methanol at up to 15% v/v Pholiota adiposa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 5.6
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 65°C Pholiota adiposa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular the enzyme is secreted Pholiota adiposa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Cu2+ strong inhibition at 5 mM Pholiota adiposa
3.2.1.21 Fe2+ 25% inhibition at 5 mM Pholiota adiposa
3.2.1.21 Hg2+ strong inhibition at 5 mM Pholiota adiposa
3.2.1.21 additional information Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effects at 5 mM. Addition of beta-mercaptoethanol or cysteine does not significantly alter activity Pholiota adiposa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 59000
-
 gel filtration Pholiota adiposa
3.2.1.21 60000
-
 1 * 60000, SDS-PAGE Pholiota adiposa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Pholiota adiposa
-
 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O Pholiota adiposa SKU0714
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Pholiota adiposa
-
-
-
3.2.1.21 Pholiota adiposa SKU0714
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein
-
 Pholiota adiposa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native extracellular enzyme 130.3fold from culture supernatant by ultrafiltration, anion exchange chromatography and gel filtration to homogeneity Pholiota adiposa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 mycelium
-
 Pholiota adiposa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2088
-
 purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 5.0, 65°C Pholiota adiposa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pholiota adiposa 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pholiota adiposa SKU0714 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-cellobioside + H2O
-
 Pholiota adiposa 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pholiota adiposa 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pholiota adiposa SKU0714 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Pholiota adiposa 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Pholiota adiposa SKU0714 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Pholiota adiposa ?
-
 ?
3.2.1.21 cellotetraose + H2O
-
 Pholiota adiposa ?
-
 ?
3.2.1.21 cellotriose + H2O
-
 Pholiota adiposa ?
-
 ?
3.2.1.21 cellotriose + H2O
-
 Pholiota adiposa SKU0714 ?
-
 ?
3.2.1.21 additional information no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside Pholiota adiposa ?
-
 ?
3.2.1.21 additional information no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside Pholiota adiposa SKU0714 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 60000, SDS-PAGE Pholiota adiposa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bgl
-
 Pholiota adiposa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65
-
-
 Pholiota adiposa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 40 70 20% of maximal activity at 40°C, 80% at 70°C, profile overview Pholiota adiposa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 3460
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 65°C Pholiota adiposa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Pholiota adiposa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3 7.5 activity range, profile overview Pholiota adiposa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 2
-
 purified enzyme, 25°C, 24 h, over 20% activity remaining Pholiota adiposa
3.2.1.21 3 6 purified enzyme, 24 h, over 80% activity remaining Pholiota adiposa
3.2.1.21 7
-
 purified enzyme, 25°C, 24 h, over 50% activity remaining Pholiota adiposa
3.2.1.21 8
-
 purified enzyme, 25°C, 24 h, over 20% activity remaining Pholiota adiposa

General Information

EC Number General Information Comment Organism
3.2.1.21 evolution the enzyme belongs to the glycoside hydrolase family 3, GH3 Pholiota adiposa