Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Masuyer, G.; Akif, M.; Czarny, B.; Beau, F.; Schwager, S.L.; Sturrock, E.D.; Isaac, R.E.; Dive, V.; Acharya, K.R.
    Crystal structures of highly specific phosphinic tripeptide enantiomers in complex with the angiotensin-I converting enzyme (2014), FEBS J., 281, 943-956.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.15.1 AnCE is cloned and expressed in Pichia pastoris Drosophila melanogaster
3.4.15.1 C-catalytic and N-catalytic domain is expressed in CHO cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.15.1 crystal structure of AnCE in complex with (2S)-2-({3-[hydroxyl (2-phenyl-(1R)-1-{[(benzyloxy) carbonyl]-amino}ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl}amino)-3-(4-hydroxy-phenyl) propanoic acid and (2R)-2-({3-[hydroxyl (2-phenyl-(1R)-1-{[(benzyloxy) carbonyl]-amino}ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl}amino)-3-(4-hydroxy-phenyl) propanoic acid are determined to a resolution of 1.8 and 2.0 A, respectively Drosophila melanogaster
3.4.15.1 crystal structures of the two catalytic domains of human ACE (N- and C-) in complex with (2S)-2-({3-[hydroxyl (2-phenyl-(1R)-1-{[(benzyloxy) carbonyl]-amino}ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl}amino)-3-(4-hydroxy-phenyl) propanoic acid and (2R)-2-({3-[hydroxyl (2-phenyl-(1R)-1-{[(benzyloxy) carbonyl]-amino}ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl}amino)-3-(4-hydroxy-phenyl) propanoic acid are determined to a resolution of 1.91 and 1.85 A, respectively Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.15.1 (2R)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid
-
 Drosophila melanogaster
3.4.15.1 (2R)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid
-
 Homo sapiens
3.4.15.1 (2S)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid
-
 Drosophila melanogaster
3.4.15.1 (2S)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.15.1 1.468
-
 hippuryl-His-Leu pH 7.5, temperature not specified in the publication Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.4.15.1 Drosophila melanogaster Q10714
-
-
3.4.15.1 Homo sapiens P12821
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.15.1 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.15.1 hippuryl-His-Leu + H2O
-
 Drosophila melanogaster hippuric acid + His-Leu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.15.1 ACE
-
 Homo sapiens
3.4.15.1 ANCE
-
 Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.15.1 7.5
-
 assay at Homo sapiens
3.4.15.1 7.5
-
 assay at Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.15.1 0.00000041
-
 (2S)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication Homo sapiens
3.4.15.1 0.00000061
-
 (2R)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication Homo sapiens
3.4.15.1 0.000024
-
 (2S)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication Drosophila melanogaster
3.4.15.1 0.00012
-
 (2R)-2-((3-[hydroxyl (2-phenyl-(1R)-1-([(benzyloxy) carbonyl]-amino)ethyl)phosphinyl]-2-[(3-phenylisoxazol-5-yl)methyl]-1-oxopropyl)amino)-3-(4-hydroxy-phenyl) propanoic acid C-catalytic domain of ACE, pH 7.5, temperature not specified in the publication Drosophila melanogaster