EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.31 | heterologous expression in Saccharomyces cerevisiae and Brassica juncea respectively. Introduction of the full-length enzyme into Brassica juncea under the control of the 35S promoter results in the production of (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaeno in leaves when exogenously supplied with (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoic acid | Thraustochytrium sp. |
1.14.19.43 | expressed in Saccharomyces cerevisiae and Brassica juncea | Thraustochytrium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.31 | 49800 | - |
x * 49800, calculated fropm sequence | Thraustochytrium sp. |
1.14.19.43 | 59100 | - |
x * 59100, calculated from amino acid sequence | Thraustochytrium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.43 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Thraustochytrium sp. | - |
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Thraustochytrium sp. ATCC 21685 | - |
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.31 | Thraustochytrium sp. | Q8S3C0 | - |
- |
1.14.19.31 | Thraustochytrium sp. ATCC 21685 | Q8S3C0 | - |
- |
1.14.19.43 | Thraustochytrium sp. | Q853C0 | - |
- |
1.14.19.43 | Thraustochytrium sp. ATCC 21685 | Q853C0 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.19.31 | a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | (1) | Thraustochytrium sp. | |
1.14.19.31 | a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | (1) | Thraustochytrium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.31 | a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Thraustochytrium sp. | a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.31 | a (7Z,10Z,13Z,16Z)-docosa-7,10,13,16-tetraenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Thraustochytrium sp. ATCC 21685 | a (4Z,7Z,10Z,13Z,16Z)-docosa-4,7,10,13,16-pentaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.31 | a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Thraustochytrium sp. | a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.31 | a (7Z,10Z,13Z,16Z,19Z)-docosa-7,10,13,16,19-pentaenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Thraustochytrium sp. ATCC 21685 | a (4Z,7Z,10Z,13Z,16Z,19Z)-docosa-4,7,10,13,16,19-hexaenoyl-[glycerolipid] + ferricytochrome b5 + 2 H2O | - |
? | |
1.14.19.43 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. | 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. ATCC 21685 | 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. | docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 22:4(n-6) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. ATCC 21685 | docosa-4,7,10,13,16-pentaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. | docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
1.14.19.43 | 22:5(n-3) + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Thraustochytrium sp. ATCC 21685 | docosa-4,7,10,13,16,19-hexaenoic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.31 | ? | x * 49800, calculated fropm sequence | Thraustochytrium sp. |
1.14.19.43 | ? | x * 59100, calculated from amino acid sequence | Thraustochytrium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.31 | DELTA4 fatty acid desaturase | - |
Thraustochytrium sp. |
1.14.19.43 | DELTA4 fatty acid desaturase | - |
Thraustochytrium sp. |
1.14.19.43 | FAD4 | - |
Thraustochytrium sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.31 | metabolism | the enzyme is involved in the biosynthesis of docosahexanoic acid | Thraustochytrium sp. |