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Literature summary extracted from

  • Fontenele, M.; Lim, B.; Oliveira, D.; Buffolo, M.; Perlman, D.H.; Schupbach, T.; Araujo, H.
    Calpain A modulates Toll responses by limited Cactus/IkappaB proteolysis (2013), Mol. Biol. Cell, 24, 2966-2980.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B36 cloning and inducible expression of C-terminally V5/His-tagged CalpA in S2 cells Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B36 NF-kappa-B inhibitor cactus negatively regulates calpain A activity. In Drosophila cactus exists as free and NFkappaB-bound forms Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B36 cytosol
-
 Drosophila melanogaster 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.B36 Ca2+ dependent on Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.B36 cactus + H2O Drosophila melanogaster an IkappaB homo­logue, Cactus and calpain A physically interact, calpain A generates a C-terminal–truncated Cactus fragment devoid of Toll-responsive sequences, cleavage analysis in vivo, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B36 Drosophila melanogaster Q11002
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B36 additional information cactus regulates calpain A localization, calpain A redistribution to the cytoplasm requires full-length cactus, overview Drosophila melanogaster
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3.4.22.B36 oocyte expression during early embryogenesis Drosophila melanogaster
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3.4.22.B36 syncytium CalpA protein is apically enriched in the submembranous cyto­plasm of the embryo syncytium Drosophila melanogaster
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B36 cactus + H2O an IkappaB homo­logue, Cactus and calpain A physically interact, calpain A generates a C-terminal–truncated Cactus fragment devoid of Toll-responsive sequences, cleavage analysis in vivo, overview Drosophila melanogaster ?
-
 ?
3.4.22.B36 cactus + H2O an IkappaB homo­logue, GFP-tagged substrate, calpain A generates a C-terminal–truncated Cactus fragment devoid of Toll-responsive sequences Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B36 CalpA
-
 Drosophila melanogaster
3.4.22.B36 calpain A
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
3.4.22.B36 additional information cactus protein levels affect the cellular localization of the enzyme, overview. Wild-type syncytial blastoderm embryo show endogenous calpain A beneath the plasma membrane, while in loss-of-function cact mutants calpain A is diffuse in the cytoplasm. In a gain-of-function cact mutant, calpain A distribution is patchy during interphase and remains so during mitosis. Cactus regulates calpain A activity and localization Drosophila melanogaster
3.4.22.B36 physiological function calpain A regulates regulate NFkappaB function by different mechanisms, it decreases IkappaB homo­logue cactus levels in vivo and regulates cactus function in the immune system. The enzyme interacts physically with cactus, recognizing a Cactus pool that is not bound to Dorsal, a fly NFkappaB/Rel homologue. The enzyme targets free cactus, which is incorporated into and modulates Toll-responsive complexes in the embryo and immune system. Effect of calpain A on the embryonic DV axis. Cactus regulates calpain A activity and localization Drosophila melanogaster