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Literature summary extracted from

  • Ghosh, A.; Pareek, A.; Sopory, S.K.; Singla-Pareek, S.L.
    A glutathione responsive rice glyoxalase II, OsGLYII-2, functions in salinity adaptation by maintaining better photosynthesis efficiency and anti-oxidant pool (2014), Plant J., 80, 93-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.6 expressed in Escherichia coli as a His-tagged fusion protein Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.6 D-lactate
-
Oryza sativa
3.1.2.6 EDTA
-
Oryza sativa
3.1.2.6 glutathione
-
Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.6 0.254
-
S-Lactoylglutathione pH and temperature not specified in the publication, Vmax: 1 mmol/min/mg Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.6 cytoplasm
-
Oryza sativa 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.2.6 Fe2+ contains a binuclear Zn/Fe centre in its active site Oryza sativa
3.1.2.6 Zn2+ contains a binuclear Zn/Fe centre in its active site Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.6 28560
-
calculated from cDNA Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.6 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.6 using Ni-NTA chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.6 anther
-
Oryza sativa
-
3.1.2.6 callus
-
Oryza sativa
-
3.1.2.6 endosperm
-
Oryza sativa
-
3.1.2.6 inflorescence
-
Oryza sativa
-
3.1.2.6 leaf
-
Oryza sativa
-
3.1.2.6 panicle
-
Oryza sativa
-
3.1.2.6 plant embryo
-
Oryza sativa
-
3.1.2.6 plant ovary
-
Oryza sativa
-
3.1.2.6 root
-
Oryza sativa
-
3.1.2.6 seedling
-
Oryza sativa
-
3.1.2.6 shoot
-
Oryza sativa
-
3.1.2.6 stigma
-
Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.6 S-lactoylglutathione + H2O
-
Oryza sativa GSH + D-lactate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.6 Gly II
-
Oryza sativa
3.1.2.6 glyoxalase II
-
Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.6 508
-
S-Lactoylglutathione pH and temperature not specified in the publication Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.6 7.2
-
-
Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.6 6 8
-
Oryza sativa

Expression

EC Number Organism Comment Expression
3.1.2.6 Oryza sativa slight downregulation during the early reproductive stage down
3.1.2.6 Oryza sativa slight upregulation during the late vegetative phase up

General Information

EC Number General Information Comment Organism
3.1.2.6 malfunction ectopic expression of OsGLYII-2 in Escherichia coli and tobacco provides improved tolerance against salinity and dicarbonyl stress indicating towards its role in abiotic stress tolerance Oryza sativa
3.1.2.6 malfunction OsGLYII-2 is able to functionally complement a yeast GLY II mutant Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.2.6 2000
-
S-Lactoylglutathione pH and temperature not specified in the publication Oryza sativa