Literature summary extracted from

Hongsthong, A.; Subudhi, S.; Sirijuntarut, M.; Kurdrid, P.; Cheevadhanarak, S.; Tanticharoen, M.
Revealing the complementation of ferredoxin by cytochrome b5 in the Spirulina-DELTA6-desaturation reaction by N-terminal fusion and co-expression of the fungal-cytochrome b5 domain and Spirulina-DELTA6-acyl-lipid desaturase (2006), Appl. Microbiol. Biotechnol., 72, 1192-1201.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.19.46	expressed as N-terminally fused and co-expressed protein with the cytochrome b5 domain from Mucor rouxii, in Escherichia coli DH5alpha cells and Saccharomyces cerevisiae strain DBY746	Arthrospira platensis

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.14.19.46	50000	-	x * 50000, SDS-PAGE	Arthrospira platensis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.19.46	1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	Arthrospira platensis	-	1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.19.46	Arthrospira platensis	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.19.46	1-linoleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+	-	Arthrospira platensis	1-gamma-linolenoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.14.19.46	?	x * 50000, SDS-PAGE	Arthrospira platensis

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.19.46	DELTA6-acyl-lipid desaturase	-	Arthrospira platensis
1.14.19.46	desD	-	Arthrospira platensis

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.19.46	Ferredoxin	-	Arthrospira platensis

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Release 2023.1 (January 2023)