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Literature summary extracted from

  • Holic, R.; Yazawa, H.; Kumagai, H.; Uemura, H.
    Engineered high content of ricinoleic acid in fission yeast Schizosaccharomyces pombe (2012), Appl. Microbiol. Biotechnol., 95, 179-187.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.18.4 synthesis at the normal growth temperature of 30°C, Schizosaccharomyces pombe cells harboring FAH12 expression vector grow poorly when the FAH12 gene expression is induced. At 37°C, there is almost no growth inhibition. After preliminary growth at 37°C followed by a 5-day incubation at 20°C , the level of ricinoleic acid reaches 137.4 microg/ml of culture which corresponds to 52.6% of total fatty acids Claviceps purpurea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.4 expression in Schizosaccharomyces pombe Claviceps purpurea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.4 Claviceps purpurea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.4 oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+
-
Claviceps purpurea ricinoleic acid + 2 ferricytochrome b5 + H2O i.e. 12-hydroxy-octadeca-cis-9-enoic acid ?

Synonyms

EC Number Synonyms Comment Organism
1.14.18.4 FAH12
-
Claviceps purpurea
1.14.18.4 oleate DELTA12-hydroxylase
-
Claviceps purpurea