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Literature summary extracted from

  • Taton, M.; Rahier, A.
    Plant sterol biosynthesis: Identification and characterization of higher plant DELTA7-sterol C5(6)-desaturase (1996), Arch. Biochem. Biophys., 325, 279-288.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.20 1,10-phenanthroline
-
 Zea mays
1.14.19.20 cyanide
-
 Zea mays
1.14.19.20 CYC (cyctochrome c)
-
 Zea mays
1.14.19.20 menadione 32% residual actitivty at 0.05 mM Zea mays
1.14.19.20 additional information not inhibited by CO Zea mays
1.14.19.20 Salicylhydroxamic acid
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.20 0.0105
-
 episterol at pH 7.6 and 30°C Zea mays
1.14.19.20 0.08
-
 stigmasta-7,22-dien-3beta-ol at pH 7.6 and 30°C Zea mays
1.14.19.20 0.115
-
 lathosterol at pH 7.6 and 30°C Zea mays
1.14.19.20 0.14
-
 avenasterol at pH 7.6 and 30°C Zea mays
1.14.19.20 0.67
-
 stigmast-7-en-3beta-ol at pH 7.6 and 30°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.20 microsome
-
 Zea mays
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.20 DELTA7-sterol + ferrocytochrome b5 + O2 + H+ Zea mays
-
 DELTA5,7-sterol + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.20 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.20 seedling
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.20 avenasterol + ferrocytochrome b5 + O2 + H+
-
 Zea mays ?
-
 ?
1.14.19.20 DELTA7-sterol + ferrocytochrome b5 + O2 + H+
-
 Zea mays DELTA5,7-sterol + ferricytochrome b5 + H2O
-
 ?
1.14.19.20 episterol + ferrocytochrome b5 + O2 + H+
-
 Zea mays ergosta-5,7,24(28)-trien-3beta-ol + ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.20 lathosterol + ferrocytochrome b5 + O2 + H+
-
 Zea mays ?
-
 ?
1.14.19.20 additional information no activity with 4alpha-methyl-5alpha-cholest-7-en-3beta-ol, 4alpha,14alpha-dimethyl-5alpha-cholest-7-en-3beta-ol, cholestan-3beta-ol and cholest-8-en-3beta-ol Zea mays ?
-
 ?
1.14.19.20 additional information the specificity of maize C5(6)-desaturase favors DELTA7-sterols possessing a C24-methylene or ethylidene substituent compared to 24-ethyl-substituted DELTA7-sterols Zea mays ?
-
 ?
1.14.19.20 stigmast-7-en-3beta-ol + ferrocytochrome b5 + O2 + H+
-
 Zea mays stigmast-5,7-dien-3beta-ol + ferricyctochrome b5 + H2O
-
 ?
1.14.19.20 stigmasta-7,22-dien-3beta-ol + ferrocytochrome b5 + O2 + H+
-
 Zea mays stigmast-5,7,22-trien-3beta-ol + ferricyctochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.20 DELTA7-sterol C56-desaturase
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.20 cytochrome b5
-
 Zea mays
1.14.19.20 NADH required, NADH is more efficient than NADPH Zea mays
1.14.19.20 NADPH NADH is more efficient than NADPH Zea mays

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.19.20 0.008
-
 at pH 7.6 and 30°C Zea mays CYC (cyctochrome c)
1.14.19.20 0.015
-
 at pH 7.6 and 30°C Zea mays cyanide
1.14.19.20 0.5
-
 at pH 7.6 and 30°C Zea mays Salicylhydroxamic acid
1.14.19.20 2
-
 at pH 7.6 and 30°C Zea mays 1,10-phenanthroline