EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.4.6 | E209K | site-directed mutagenesis | Drosophila melanogaster |
3.6.4.6 | additional information | construction of Vps4 knockout mutants, loss of Vps4 affects multiple signaling pathways, phenotypes, detailed overview | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.4.6 | membrane | - |
Drosophila melanogaster | 16020 | - |
3.6.4.6 | vesicle | intra-lumenal | Drosophila melanogaster | 31982 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.4.6 | Mg2+ | required | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.6 | ATP + H2O | Drosophila melanogaster | - |
ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.4.6 | Drosophila melanogaster | Q9Y162 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.4.6 | eye | - |
Drosophila melanogaster | - |
3.6.4.6 | ovary | follicle cell | Drosophila melanogaster | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.4.6 | ATP + H2O | - |
Drosophila melanogaster | ADP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.4.6 | hexamer | - |
Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.4.6 | vacuolar protein sorting 4 | - |
Drosophila melanogaster |
3.6.4.6 | Vps4 | - |
Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.4.6 | evolution | enzyme vacuolar protein sorting 4, Vps4, belongs to the AAA ATPases. Vps4 forms a hexameric complex that disassembles ESCRT-III, allowing recycling of its components, and also plays an active role in scission of the vesicle neck. Vps4 is required for epidermal growth factor receptor signaling even in the absence of Shibire, the Dynamin that internalizes epidermal growth factor receptor from the plasma membrane. In ovarian follicle cells, in contrast, Vps4 does not affect epidermal growth factor receptor signaling, although it is still essential for receptor degradation, Vps4 is not required for EGFR or Notch signaling in follicle cells. Enzyme Vps4 can promote epidermal growth factor receptor activity through an endocytosis-independent mechanism, a non-endocytic and cell type-dependent mechanism. Protein Vps4 is the only energy-utilizing ESCRT component | Drosophila melanogaster |
3.6.4.6 | malfunction | photoreceptors are largely absent from Vps4 mutant clones in the eye disc, the resulting mutant R8 photoreceptors fail to recruit surrounding cells to differentiate as R1-R7 photoreceptors. Loss of Vps4 disrupts the epidermal growth factor receptor, EGFR, pathway. In imaginal disc cells deficient for enzyme Vps4, EGFR and other receptors accumulate in endosomes, and EGFR target genes are not expressed | Drosophila melanogaster |
3.6.4.6 | physiological function | enzyme vacuolar protein sorting 4, Vps4, interacts with the endosomal sorting complexes required for transport, ESCRT-III, complex to drive the final step of intra-lumenal vesicles formation | Drosophila melanogaster |