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Literature summary extracted from

  • Stuer-Lauridsen, B.; Nygaard, P.
    Purine salvage in two halophilic archaea: characterization of salvage pathways and isolation of mutants resistant to purine analogs (1998), J. Bacteriol., 180, 457-463.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Halobacterium salinarum
-
-
-
2.4.2.1 Haloferax volcanii
-
-
-
2.4.2.7 Halobacterium salinarum
-
-
-
2.4.2.7 Haloferax volcanii
-
-
-
2.4.2.8 Halobacterium salinarum
-
-
-
2.4.2.8 Haloferax volcanii
-
-
-
2.7.1.73 Halobacterium salinarum
-
-
-
2.7.1.73 Haloferax volcanii
-
-
-
3.5.4.3 Halobacterium salinarum
-
-
-
3.5.4.3 Haloferax volcanii
-
-
-
3.5.4.4 Halobacterium salinarum
-
-
-
3.5.4.4 Haloferax volcanii
-
-
-
4.3.2.2 Halobacterium salinarum
-
-
-
4.3.2.2 Haloferax volcanii
-
-
-
6.3.4.4 Halobacterium salinarum
-
-
-
6.3.4.4 Haloferax volcanii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 10
-
 substrate adenosine, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.1 370
-
 substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.1 2630
-
 substrate adenosine, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.4.2.1 2890
-
 substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.1 3740
-
 substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.4.2.1 5420
-
 substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.4.2.7 1430
-
 crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.4.2.7 2050
-
 crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.8 870
-
 substrate GMP, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.8 2130
-
 substrate IMP, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.4.2.8 2340
-
 substrate IMP, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.4.2.8 2450
-
 substrate GMP, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.7.1.73 50
-
 substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.7.1.73 60
-
 substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
2.7.1.73 120
-
 substrate guanosine, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
2.7.1.73 120
-
 substrate inosine, crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
3.5.4.3 530
-
 crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
3.5.4.3 1140
-
 crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
3.5.4.4 120
-
 crude cell extract, pH 7.5, temperature not specified in the publication Haloferax volcanii
3.5.4.4 760
-
 crude cell extract, pH 7.5, temperature not specified in the publication Halobacterium salinarum
4.3.2.2 20
-
 crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication Haloferax volcanii
4.3.2.2 40
-
 crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication Halobacterium salinarum
6.3.4.4 560
-
 crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication Halobacterium salinarum
6.3.4.4 720
-
 crude cell extract, presence of 1 M KCl, pH 7.5, temperature not specified in the publication Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Halobacterium salinarum adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate
-
 Haloferax volcanii adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Halobacterium salinarum guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Haloferax volcanii guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Halobacterium salinarum hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Haloferax volcanii hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.7 AMP + diphosphate
-
 Halobacterium salinarum adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.7 AMP + diphosphate
-
 Haloferax volcanii adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 GMP + diphosphate
-
 Halobacterium salinarum guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 GMP + diphosphate
-
 Haloferax volcanii guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 IMP + diphosphate
-
 Halobacterium salinarum hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.4.2.8 IMP + diphosphate
-
 Haloferax volcanii hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.1.73 ATP + guanosine
-
 Halobacterium salinarum ADP + GMP
-
 ?
2.7.1.73 ATP + guanosine
-
 Haloferax volcanii ADP + GMP
-
 ?
2.7.1.73 ATP + inosine
-
 Halobacterium salinarum ADP + IMP
-
 ?
2.7.1.73 ATP + inosine
-
 Haloferax volcanii ADP + IMP
-
 ?
3.5.4.3 guanine + H2O
-
 Halobacterium salinarum xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Haloferax volcanii xanthine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Halobacterium salinarum inosine + NH3
-
 ?
3.5.4.4 adenosine + H2O
-
 Haloferax volcanii inosine + NH3
-
 ?
4.3.2.2 adenylosuccinate
-
 Halobacterium salinarum adenosine monophosphate + fumarate
-
 ?
4.3.2.2 adenylosuccinate
-
 Haloferax volcanii adenosine monophosphate + fumarate
-
 ?
6.3.4.4 ATP + IMP + L-Asp
-
 Halobacterium salinarum GDP + phosphate + adenylosuccinate
-
 ?
6.3.4.4 ATP + IMP + L-Asp
-
 Haloferax volcanii GDP + phosphate + adenylosuccinate
-
 ?

General Information

EC Number General Information Comment Organism
2.4.2.1 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
2.4.2.1 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
2.4.2.7 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
2.4.2.7 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
2.4.2.8 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
2.4.2.8 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
2.7.1.73 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
2.7.1.73 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
3.5.4.3 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
3.5.4.3 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
3.5.4.4 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
3.5.4.4 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
4.3.2.2 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
4.3.2.2 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii
6.3.4.4 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Halobacterium salinarum
6.3.4.4 metabolism comparison of growth characteristics including intracellular protein levels, RNA content, and nucleotide pool sizes between the extreme halophile Halobacterium halobium and the moderate halophile Haloferax volcanii. The differences in the metabolism of purine bases and nucleosides and the sensitivity to purine analogs between the two halobacteria are reflected in differences in purine enzyme levels Haloferax volcanii