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Literature summary extracted from

  • Garcia-Fontana, C.; Reyes-Darias, J.A.; Munoz-Martinez, F.; Alfonso, C.; Morel, B.; Ramos, J.L.; Krell, T.
    High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation (2013), J. Biol. Chem., 288, 18987-18999.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.80 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida
2.1.1.80 expressed in Escherichia coli C41(DE3) cells Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.80 32247
-
 1 * 32247, isoform CheR2, calculated from amino acid sequence Pseudomonas putida
2.1.1.80 38600
-
 1 * 38600, isoform CheR2, sedimentation equilibrium ultracentrifugation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate Pseudomonas putida substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate Pseudomonas putida KT 2240 substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate Pseudomonas putida substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate Pseudomonas putida KT 2240 substrate for isoform CheR2 only S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.80 Pseudomonas putida Q88ER1
-
-
2.1.1.80 Pseudomonas putida Q88GG4
-
-
2.1.1.80 Pseudomonas putida Q88MS8
-
-
2.1.1.80 Pseudomonas putida KT 2240 Q88ER1
-
-
2.1.1.80 Pseudomonas putida KT 2240 Q88GG4
-
-
2.1.1.80 Pseudomonas putida KT 2240 Q88MS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.80 HisTrap column chromatography Pseudomonas putida
2.1.1.80 HisTrap column chromatography, and gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.80 additional information no activity with chemotaxis receptors McpS and chemotaxis receptors McpT Pseudomonas putida ?
-
 ?
2.1.1.80 additional information no activity with chemotaxis receptors McpS and chemotaxis receptors McpT Pseudomonas putida KT 2240 ?
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate substrate for isoform CheR2 only Pseudomonas putida S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate substrate for isoform CheR2 only Pseudomonas putida KT 2240 S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate substrate for isoform CheR2 only Pseudomonas putida S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?
2.1.1.80 S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate substrate for isoform CheR2 only Pseudomonas putida KT 2240 S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.80 monomer 1 * 32247, isoform CheR2, calculated from amino acid sequence Pseudomonas putida
2.1.1.80 monomer 1 * 38600, isoform CheR2, sedimentation equilibrium ultracentrifugation Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
2.1.1.80 CheR1 isoform Pseudomonas putida
2.1.1.80 CheR2 isoform Pseudomonas putida
2.1.1.80 CheR3 isoform Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.80 44
-
 isoform CheR1 has a melting temperature of 44°C Pseudomonas putida
2.1.1.80 48
-
 isoform CheR2 has a melting temperature of 48°C Pseudomonas putida
2.1.1.80 49
-
 isoform CheR3 has a melting temperature of 49°C Pseudomonas putida

General Information

EC Number General Information Comment Organism
2.1.1.80 physiological function isoform CheR1 is required for efficient biofilm formation Pseudomonas putida
2.1.1.80 physiological function isoform CheR2 is essential for chemotaxis Pseudomonas putida