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Literature summary extracted from

  • Michaelson, L.; Markham, J.; Zäeuner, S.; Matsumoto, M.; Chen, M.; Cahoon, E.; Napier, J.
    Identification of a cytochrome b5-fusion desaturase responsible for the synthesis of triunsaturated sphingolipid long chain bases in the marine diatom Thalassiosira pseudonana (2013), Phytochemistry, 90, 50-55.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.19 expressed in Yarrowia lipolytica strain ATCC 90811 and in Pichia pastoris strain PpDELTAc9m Thalassiosira pseudonana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + ferrocytochrome b5 + O2 + H+ Thalassiosira pseudonana
-
 (4E,8E,10E)-sphinga-4,8,10-trienine ceramide + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.19 Thalassiosira pseudonana Q4G2T3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.19 (4E,8E)-sphinga-4,8-dienine ceramide + ferrocytochrome b5 + O2 + H+
-
 Thalassiosira pseudonana (4E,8E,10E)-sphinga-4,8,10-trienine ceramide + ferricytochrome b5 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.19 DesA
-
 Thalassiosira pseudonana
1.14.19.19 diene DELTA10-desaturase
-
 Thalassiosira pseudonana
1.14.19.19 sphingolipid DELTA10-desaturase
-
 Thalassiosira pseudonana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.19 cytochrome b5
-
 Thalassiosira pseudonana